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Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
M. Tvrdonova, M. Vlcnovska, LP. Vanickova, V. Kanicky, V. Adam, L. Ascher, N. Jakubowski, M. Vaculovicova, T. Vaculovic,
Language English Country Germany
Document type Journal Article
NLK
ProQuest Central
from 2011-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2003-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2011-01-01 to 1 year ago
- MeSH
- Mass Spectrometry methods MeSH
- Immunoassay methods MeSH
- Indicators and Reagents chemistry MeSH
- Metal Nanoparticles chemistry MeSH
- Lasers * MeSH
- Limit of Detection MeSH
- Proof of Concept Study MeSH
- Antibodies chemistry MeSH
- Antibody Specificity MeSH
- Gold chemistry MeSH
- Publication type
- Journal Article MeSH
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
References provided by Crossref.org
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- $a Tvrdonova, Michaela $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. CEITEC, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
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- $a Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry / $c M. Tvrdonova, M. Vlcnovska, LP. Vanickova, V. Kanicky, V. Adam, L. Ascher, N. Jakubowski, M. Vaculovicova, T. Vaculovic,
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- $a In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
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- $a Vlcnovska, Marcela $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.
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- $a Vanickova, Lucie Pompeiano $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
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- $a Kanicky, Viktor $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. CEITEC, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
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- $a Vaculovic, Tomas $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. vaca_777@yahoo.com. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic. vaca_777@yahoo.com.
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