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Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
M. Tvrdonova, M. Vlcnovska, LP. Vanickova, V. Kanicky, V. Adam, L. Ascher, N. Jakubowski, M. Vaculovicova, T. Vaculovic,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 2011-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2011-01-01 do Před 1 rokem
- MeSH
- hmotnostní spektrometrie metody MeSH
- imunoanalýza metody MeSH
- indikátory a reagencie chemie MeSH
- kovové nanočástice chemie MeSH
- lasery * MeSH
- limita detekce MeSH
- ověření koncepční studie MeSH
- protilátky chemie MeSH
- specificita protilátek MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
Citace poskytuje Crossref.org
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- $a Tvrdonova, Michaela $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. CEITEC, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
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- $a Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry / $c M. Tvrdonova, M. Vlcnovska, LP. Vanickova, V. Kanicky, V. Adam, L. Ascher, N. Jakubowski, M. Vaculovicova, T. Vaculovic,
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- $a In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
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- $a Vlcnovska, Marcela $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.
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- $a Kanicky, Viktor $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. CEITEC, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
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- $a Adam, Vojtech $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
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- $a Ascher, Lena $u Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
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- $a Vaculovic, Tomas $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic. vaca_777@yahoo.com. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic. vaca_777@yahoo.com.
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