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Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme

L. Grillová, T. Bawa, L. Mikalová, A. Gayet-Ageron, K. Nieselt, M. Strouhal, P. Sednaoui, T. Ferry, M. Cavassini, S. Lautenschlager, F. Dutly, M. Pla-Díaz, M. Krützen, F. González-Candelas, HC. Bagheri, D. Šmajs, N. Arora, PP. Bosshard,

. 2018 ; 13 (7) : e0200773. [pub] 20180730

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19012513

Grantová podpora
NV17-31333A MZ0 CEP - Centrální evidence projektů

Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.

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$a Grillová, Linda $u Department of Biology, Masaryk University, Brno, Czech Republic.
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$a Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.
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$a Bawa, Tanika $u Department of Fundamental Neuroscience, University of Geneva, Geneva, Switzerland.
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$a Mikalová, Lenka $u Department of Biology, Masaryk University, Brno, Czech Republic.
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$a Gayet-Ageron, Angèle $u Division of Clinical Epidemiology, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
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$a Nieselt, Kay $u Center for Bioinformatics, University of Tübingen, Tübingen, Germany.
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$a Strouhal, Michal $u Department of Biology, Masaryk University, Brno, Czech Republic.
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$a Sednaoui, Patrice $u Medical Biology, Institute Alfred Fournier, Paris, France.
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$a Ferry, Tristan $u Hospices civils de Lyon, Lyon, France.
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$a Cavassini, Matthias $u Department of Infectious Diseases, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
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$a Lautenschlager, Stephan $u Department of Dermatology, Triemlispital, Zurich, Switzerland.
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$a Dutly, Fabrizio $u IMD Institut für medizinische & molekulare Diagnostik AG, Zurich, Switzerland.
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$a Pla-Díaz, Marta $u Unidad Mixta Infección y Salud Pública FISABIO/Universidad de Valencia, CIBER in Epidemiology and Public Health, Valencia, Spain.
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$a Krützen, Michael $u Department of Anthropology, University of Zurich, Zurich, Switzerland.
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$a González-Candelas, Fernando $u Unidad Mixta Infección y Salud Pública FISABIO/Universidad de Valencia, CIBER in Epidemiology and Public Health, Valencia, Spain.
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$a Bagheri, Homayoun C $u Repsol Technology Center, Madrid, Spain.
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$a Šmajs, David $u Department of Biology, Masaryk University, Brno, Czech Republic.
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$a Arora, Natasha $u Institute for Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland. Zurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland.
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$a Bosshard, Philipp P $u Department of Dermatology, University Hospital Zurich, Zurich, Switzerland. Faculty of Medicine, University of Zurich, Zurich, Switzerland.
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