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An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones
O. Novák, K. Floková,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cyclopentanes analysis MeSH
- Abscisic Acid analysis MeSH
- Salicylic Acid analysis MeSH
- Indoleacetic Acids analysis MeSH
- Oxylipins analysis MeSH
- Plant Growth Regulators analysis MeSH
- Tandem Mass Spectrometry methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
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- $a The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
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- $a Floková, Kristýna $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic. Faculty of Science, Palacký University, Olomouc, Czech Republic. Plant Hormone Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
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