-
Je něco špatně v tomto záznamu ?
The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin
J. Kotál, N. Stergiou, M. Buša, A. Chlastáková, Z. Beránková, P. Řezáčová, H. Langhansová, A. Schwarz, E. Calvo, J. Kopecký, M. Mareš, E. Schmitt, J. Chmelař, M. Kotsyfakis,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Grantová podpora
RVO 60077344
Parazitologická ústav, Akademie Věd České Republiky
RVO 61388963
Ústav Organické Chemie a Biochemie, Akademie Věd České Republiky
NLK
PubMed Central
od 1997
ProQuest Central
od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-01-01 do Před 1 rokem
- MeSH
- cystatiny klasifikace genetika farmakologie MeSH
- cytokiny metabolismus MeSH
- epoxidové sloučeniny metabolismus MeSH
- fylogeneze MeSH
- imunosupresiva chemie metabolismus farmakologie MeSH
- klíště chemie genetika metabolismus MeSH
- krystalografie rentgenová MeSH
- makrofágy účinky léků metabolismus MeSH
- oxid dusnatý metabolismus MeSH
- proteiny členovců chemie genetika farmakologie MeSH
- proteolýza účinky léků MeSH
- sekvence aminokyselin MeSH
- sekvenční homologie aminokyselin MeSH
- slinné cystatiny chemie genetika farmakologie MeSH
- T-lymfocyty účinky léků metabolismus MeSH
- tyrosin analogy a deriváty metabolismus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
To successfully feed, ticks inject pharmacoactive molecules into the vertebrate host including cystatin cysteine protease inhibitors. However, the molecular and cellular events modulated by tick saliva remain largely unknown. Here, we describe and characterize a novel immunomodulatory cystatin, Iristatin, which is upregulated in the salivary glands of feeding Ixodes ricinus ticks. We present the crystal structure of Iristatin at 1.76 Å resolution. Purified recombinant Iristatin inhibited the proteolytic activity of cathepsins L and C and diminished IL-2, IL-4, IL-9, and IFN-γ production by different T-cell populations, IL-6 and IL-9 production by mast cells, and nitric oxide production by macrophages. Furthermore, Iristatin inhibited OVA antigen-induced CD4+ T-cell proliferation and leukocyte recruitment in vivo and in vitro. Our results indicate that Iristatin affects wide range of anti-tick immune responses in the vertebrate host and may be exploitable as an immunotherapeutic.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19027822
- 003
- CZ-PrNML
- 005
- 20240904095948.0
- 007
- ta
- 008
- 190813s2019 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s00018-019-03034-3 $2 doi
- 035 __
- $a (PubMed)30747251
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Kotál, Jan $u Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre CAS, Institute of Parasitology, Branišovská 1160/31, 37005, České Budějovice, Czech Republic. Department of Medical Biology, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, 37005, České Budějovice, Czech Republic.
- 245 14
- $a The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin / $c J. Kotál, N. Stergiou, M. Buša, A. Chlastáková, Z. Beránková, P. Řezáčová, H. Langhansová, A. Schwarz, E. Calvo, J. Kopecký, M. Mareš, E. Schmitt, J. Chmelař, M. Kotsyfakis,
- 520 9_
- $a To successfully feed, ticks inject pharmacoactive molecules into the vertebrate host including cystatin cysteine protease inhibitors. However, the molecular and cellular events modulated by tick saliva remain largely unknown. Here, we describe and characterize a novel immunomodulatory cystatin, Iristatin, which is upregulated in the salivary glands of feeding Ixodes ricinus ticks. We present the crystal structure of Iristatin at 1.76 Å resolution. Purified recombinant Iristatin inhibited the proteolytic activity of cathepsins L and C and diminished IL-2, IL-4, IL-9, and IFN-γ production by different T-cell populations, IL-6 and IL-9 production by mast cells, and nitric oxide production by macrophages. Furthermore, Iristatin inhibited OVA antigen-induced CD4+ T-cell proliferation and leukocyte recruitment in vivo and in vitro. Our results indicate that Iristatin affects wide range of anti-tick immune responses in the vertebrate host and may be exploitable as an immunotherapeutic.
- 650 _2
- $a sekvence aminokyselin $7 D000595
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a proteiny členovců $x chemie $x genetika $x farmakologie $7 D060829
- 650 _2
- $a krystalografie rentgenová $7 D018360
- 650 _2
- $a cystatiny $x klasifikace $x genetika $x farmakologie $7 D015891
- 650 _2
- $a cytokiny $x metabolismus $7 D016207
- 650 _2
- $a epoxidové sloučeniny $x metabolismus $7 D004852
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a imunosupresiva $x chemie $x metabolismus $x farmakologie $7 D007166
- 650 _2
- $a klíště $x chemie $x genetika $x metabolismus $7 D018884
- 650 _2
- $a makrofágy $x účinky léků $x metabolismus $7 D008264
- 650 _2
- $a oxid dusnatý $x metabolismus $7 D009569
- 650 _2
- $a fylogeneze $7 D010802
- 650 _2
- $a proteolýza $x účinky léků $7 D059748
- 650 _2
- $a slinné cystatiny $x chemie $x genetika $x farmakologie $7 D055333
- 650 _2
- $a sekvenční homologie aminokyselin $7 D017386
- 650 _2
- $a T-lymfocyty $x účinky léků $x metabolismus $7 D013601
- 650 _2
- $a tyrosin $x analogy a deriváty $x metabolismus $7 D014443
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Stergiou, Natascha $u Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstrasse 1, Mainz, 55131, Germany.
- 700 1_
- $a Buša, Michal $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610, Prague, Czech Republic. $7 xx0321915
- 700 1_
- $a Chlastáková, Adéla $u Department of Medical Biology, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, 37005, České Budějovice, Czech Republic.
- 700 1_
- $a Beránková, Zuzana $u Department of Medical Biology, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, 37005, České Budějovice, Czech Republic.
- 700 1_
- $a Řezáčová, Pavlína $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610, Prague, Czech Republic.
- 700 1_
- $a Langhansová, Helena $u Department of Medical Biology, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, 37005, České Budějovice, Czech Republic.
- 700 1_
- $a Schwarz, Alexandra $u Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre CAS, Institute of Parasitology, Branišovská 1160/31, 37005, České Budějovice, Czech Republic.
- 700 1_
- $a Calvo, Eric $u Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD, 20852, USA.
- 700 1_
- $a Kopecký, Jan $u Department of Medical Biology, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, 37005, České Budějovice, Czech Republic.
- 700 1_
- $a Mareš, Michael $u Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610, Prague, Czech Republic.
- 700 1_
- $a Schmitt, Edgar $u Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstrasse 1, Mainz, 55131, Germany.
- 700 1_
- $a Chmelař, Jindřich $u Department of Medical Biology, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, 37005, České Budějovice, Czech Republic.
- 700 1_
- $a Kotsyfakis, Michail $u Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre CAS, Institute of Parasitology, Branišovská 1160/31, 37005, České Budějovice, Czech Republic. mich_kotsyfakis@yahoo.com.
- 773 0_
- $w MED00001078 $t Cellular and molecular life sciences $x 1420-9071 $g Roč. 76, č. 10 (2019), s. 2003-2013
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30747251 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190813 $b ABA008
- 991 __
- $a 20240904095943 $b ABA008
- 999 __
- $a ok $b bmc $g 1432971 $s 1066282
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 76 $c 10 $d 2003-2013 $e 20190212 $i 1420-9071 $m Cellular and molecular life sciences $n Cell Mol Life Sci $x MED00001078
- GRA __
- $a RVO 60077344 $p Parazitologická ústav, Akademie Věd České Republiky
- GRA __
- $a RVO 61388963 $p Ústav Organické Chemie a Biochemie, Akademie Věd České Republiky
- LZP __
- $a Pubmed-20190813
