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Needleless electrospun and centrifugal spun poly-ε-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs
V. Lukášová, M. Buzgo, K. Vocetková, V. Sovková, M. Doupník, E. Himawan, A. Staffa, R. Sedláček, H. Chlup, F. Rustichelli, E. Amler, M. Rampichová,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
NV17-32285A
MZ0
CEP - Centrální evidence projektů
NV17-32285A
MZ0
CEP - Centrální evidence projektů
- MeSH
- alkalická fosfatasa metabolismus MeSH
- buněčná adheze MeSH
- buněčná diferenciace MeSH
- buněčné kultury MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie metabolismus MeSH
- modul pružnosti MeSH
- osteogeneze MeSH
- polyestery chemie MeSH
- poréznost MeSH
- proliferace buněk MeSH
- tkáňové inženýrství * MeSH
- tkáňové podpůrné struktury chemie MeSH
- trombocyty cytologie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The biofunctionalization of scaffolds for tissue engineering is crucial to improve the results of regenerative therapies. This study compared the effect of platelet-functionalization of 2D electrospun and 3D centrifugal spun scaffolds on the osteogenic potential of hMSCs. Scaffolds prepared from poly-ε-caprolactone, using electrospinning and centrifugal spinning technology, were functionalized using five different concentrations of platelets. Cell proliferation, metabolic activity and osteogenic differentiation were tested using hMSCs cultured in differential and non-differential medium. The porous 3D structure of the centrifugal spun fibers resulted in higher cell proliferation. Furthermore, the functionalization of the scaffolds with platelets resulted in a dose-dependent increase in cell metabolic activity, proliferation and production of an osteogenic marker - alkaline phosphatase. The effect was further promoted by culture in an osteogenic differential medium. The increase in combination of both platelets and osteogenic media shows an improved osteoinduction by platelets in environments rich in inorganic phosphate and ascorbate. Nevertheless, the results of the study showed that the optimal concentration of platelets for induction of hMSC osteogenesis is in the range of 900-3000 × 109 platelets/L. The study determines the potential of electrospun and centrifugal spun fibers with adhered platelets, for use in bone tissue engineering.
Citace poskytuje Crossref.org
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- $a Lukášová, V $u University Center for Energy Efficient Buildings (UCEEB), Czech Technical University in Prague, Třinecká 1024, 273 43, Buštěhrad, Czech Republic; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 40 Prague, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Albertov 6, 128 43 Prague, Czech Republic.
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- $a The biofunctionalization of scaffolds for tissue engineering is crucial to improve the results of regenerative therapies. This study compared the effect of platelet-functionalization of 2D electrospun and 3D centrifugal spun scaffolds on the osteogenic potential of hMSCs. Scaffolds prepared from poly-ε-caprolactone, using electrospinning and centrifugal spinning technology, were functionalized using five different concentrations of platelets. Cell proliferation, metabolic activity and osteogenic differentiation were tested using hMSCs cultured in differential and non-differential medium. The porous 3D structure of the centrifugal spun fibers resulted in higher cell proliferation. Furthermore, the functionalization of the scaffolds with platelets resulted in a dose-dependent increase in cell metabolic activity, proliferation and production of an osteogenic marker - alkaline phosphatase. The effect was further promoted by culture in an osteogenic differential medium. The increase in combination of both platelets and osteogenic media shows an improved osteoinduction by platelets in environments rich in inorganic phosphate and ascorbate. Nevertheless, the results of the study showed that the optimal concentration of platelets for induction of hMSC osteogenesis is in the range of 900-3000 × 109 platelets/L. The study determines the potential of electrospun and centrifugal spun fibers with adhered platelets, for use in bone tissue engineering.
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