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MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

A. Kopkova, J. Sana, P. Fadrus, T. Machackova, M. Vecera, V. Vybihal, J. Juracek, P. Vychytilova-Faltejskova, M. Smrcka, O. Slaby,

. 2018 ; 13 (12) : e0208580. [pub] 20181207

Language English Country United States

Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19028005

Grant support
NV15-34553A MZ0 CEP Register

Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.

References provided by Crossref.org

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$a Fadrus, Pavel $u Department of Neurosurgery, University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
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$a Vecera, Marek $u Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
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$a Vybihal, Vaclav $u Department of Neurosurgery, University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
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