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High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by "Candidatus Phytoplasma prunorum"
A. Eichmeier, T. Kiss, T. Necas, E. Penazova, D. Tekielska, M. Bohunicka, L. Valentova, R. Cmejla, D. Morais, P. Baldrian,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
QJ1510352
Ministry of Agriculture of the Czech Republic
CZ.02.1.01/0.0/0.0/16_025/0007314
Ministerstvo Školství, Mládeže a Tělovýchovy
LM2015042
Ministerstvo Školství, Mládeže a Tělovýchovy
NLK
ProQuest Central
od 2000-11-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2000-11-01 do Před 1 rokem
- MeSH
- Bacteria klasifikace genetika izolace a purifikace MeSH
- biodiverzita MeSH
- floém mikrobiologie MeSH
- nemoci rostlin mikrobiologie MeSH
- ovoce mikrobiologie MeSH
- Phytoplasma fyziologie MeSH
- slivoň mikrobiologie MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- Publikační typ
- časopisecké články MeSH
"Candidatus Phytoplasma prunorum" (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real-time PCR assays were used to select CPp-positive (group A) and CPp-negative samples (group B). Afterwards, high-throughput amplicon sequencing was performed to assess bacterial community compositions in phloem tissues. The bacterial composition in phloem tissue consisted of 118 distinct genera, represented mainly by Pseudomonas, Acinetobacter, Methylobacterium, Sphingomonas, and Rhizobium. Statistics showed that CPp influenced the bacterial composition of infected plants (group A) and that the bacterial community depended on the geographical origin of the sample. This is the first work focusing on an analysis of the influence of CPp on the bacteria coexisting in the phloem tissues of stone fruit trees.
Department of Fruit Growing Mendel University in Brno Valtická 337 691 44 Lednice Czech Republic
Mendeleum Institute of Genetics Mendel University in Brno Valtická 334 691 44 Lednice Czech Republic
Research and Breeding Institute of Pomology Holovousy Ltd Holovousy 129 508 01 Hořice Czech Republic
Citace poskytuje Crossref.org
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