The purpose of this study was to determine whether oral mucosa wounds in rats can be successfully treated with triamcinolone acetonide (TA), incorporated into liposomes. A round wound was inflicted on the oral mucosa of female Wistar rats divided into four groups of 12 animals. This wound was treated topically from day 1 with liposomes without the inclusion of TA and liposomes containing 0.01% or 0.05% TA. The wounds of the animals in the control group were not treated. Polymethyl metacrylate was used as an ointment for mixing in liposomes. The size of the wound was measured until day 6. The area of inflammatory infiltrate under the wound was evaluated by histopathology, the expression of iNOS (inducible nitric oxide synthase) enzyme under the wound was evaluated by immunohistochemistry until day 6. On the sixth day of experiment, the size of the wound and the area of the inflammatory infiltrate was the smallest in the group receiving empty liposomes (EL). Expression of iNOS was the most reduced in the group receiving EL. We conclude that oral mucosa wounds can be successfully treated with liposomes, although the incorporated drug triamcinolone would not be the appropriate drug for treating wounds of traumatic origin.

University of Ljubljana Faculty of Medicine Department of Oral Medicine and Periodontology Ljubljana Slovenia

University of Ljubljana Veterinary Faculty Small animal clinic Ljubljana Slovenia

University of Veterinary and Pharmaceutical Sciences Brno Faculty of Veterinary Medicine Small Animal Clinic Brno Czech Republic

Literatura

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$a Erjavec, Vladimira $u University of Ljubljana, Veterinary Faculty, Small animal clinic, Ljubljana, Slovenia

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$a The effect of triamcinolone in liposomes on oral wound healing in rats / $c Vladimira Erjavec, Zlatko Pavlica, Tomáš Fichtel, Milan Petelin

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$a The purpose of this study was to determine whether oral mucosa wounds in rats can be successfully treated with triamcinolone acetonide (TA), incorporated into liposomes. A round wound was inflicted on the oral mucosa of female Wistar rats divided into four groups of 12 animals. This wound was treated topically from day 1 with liposomes without the inclusion of TA and liposomes containing 0.01% or 0.05% TA. The wounds of the animals in the control group were not treated. Polymethyl metacrylate was used as an ointment for mixing in liposomes. The size of the wound was measured until day 6. The area of inflammatory infiltrate under the wound was evaluated by histopathology, the expression of iNOS (inducible nitric oxide synthase) enzyme under the wound was evaluated by immunohistochemistry until day 6. On the sixth day of experiment, the size of the wound and the area of the inflammatory infiltrate was the smallest in the group receiving empty liposomes (EL). Expression of iNOS was the most reduced in the group receiving EL. We conclude that oral mucosa wounds can be successfully treated with liposomes, although the incorporated drug triamcinolone would not be the appropriate drug for treating wounds of traumatic origin.

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