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A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane

M. Bacakova, J. Pajorova, A. Broz, D. Hadraba, F. Lopot, A. Zavadakova, L. Vistejnova, M. Beno, I. Kostic, V. Jencova, L. Bacakova,

. 2019 ; 14 (-) : 5033-5050. [pub] 20190708

Jazyk angličtina

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19034438

Background: Repairs to deep skin wounds continue to be a difficult issue in clinical practice. A promising approach is to fabricate full-thickness skin substitutes with functions closely similar to those of the natural tissue. For many years, a three-dimensional (3D) collagen hydrogel has been considered to provide a physiological 3D environment for co-cultivation of skin fibroblasts and keratinocytes. This collagen hydrogel is frequently used for fabricating tissue-engineered skin analogues with fibroblasts embedded inside the hydrogel and keratinocytes cultivated on its surface. Despite its unique biological properties, the collagen hydrogel has insufficient stiffness, with a tendency to collapse under the traction forces generated by the embedded cells. Methods: The aim of our study was to develop a two-layer skin construct consisting of a collagen hydrogel reinforced by a nanofibrous poly-L-lactide (PLLA) membrane pre-seeded with fibroblasts. The attractiveness of the membrane for dermal fibroblasts was enhanced by coating it with a thin nanofibrous fibrin mesh. Results: The fibrin mesh promoted the adhesion, proliferation and migration of the fibroblasts upwards into the collagen hydrogel. Moreover, the fibroblasts spontaneously migrating into the collagen hydrogel showed a lower tendency to contract and shrink the hydrogel by their traction forces. The surface of the collagen was seeded with human dermal keratinocytes. The keratinocytes were able to form a basal layer of highly mitotically-active cells, and a suprabasal layer. Conclusion: The two-layer skin construct based on collagen hydrogel with spontaneously immigrated fibroblasts and reinforced by a fibrin-coated nanofibrous membrane seems to be promising for the construction of full-thickness skin substitute.

Citace poskytuje Crossref.org

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$a Pajorova, Julia $u Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. 2nd Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Broz, Antonin $u Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
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$a Hadraba, Daniel $u Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic.
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$a Lopot, Frantisek $u Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic.
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$a Zavadakova, Anna $u Biomedical Center, Medical Faculty in Pilsen, Charles University, Pilsen, Czech Republic.
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$a Vistejnova, Lucie $u Biomedical Center, Medical Faculty in Pilsen, Charles University, Pilsen, Czech Republic.
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$a Beno, Milan $u Institute of Experimental Endocrinology, Biomedical Research Center of the Slovak Academy of Sciences, Bratislava, Slovak Republic.
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$a Kostic, Ivan $u Institute of Informatics, Slovak Academy of Sciences, Bratislava, Slovak Republic.
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$a Jencova, Vera $u Department of Chemistry, Technical University of Liberec, Liberec, Czech Republic.
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