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Transcriptome, proteome and draft genome of Euglena gracilis

TE. Ebenezer, M. Zoltner, A. Burrell, A. Nenarokova, AMG. Novák Vanclová, B. Prasad, P. Soukal, C. Santana-Molina, E. O'Neill, NN. Nankissoor, N. Vadakedath, V. Daiker, S. Obado, S. Silva-Pereira, AP. Jackson, DP. Devos, J. Lukeš, M. Lebert, S....

. 2019 ; 17 (1) : 11. [pub] 20190207

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19034778

Grantová podpora
P009018/1 Medical Research Council - United Kingdom

BACKGROUND: Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in a range of harsh environments. E. gracilis has been a popular model organism and of considerable biotechnological interest, but the absence of a gene catalogue has hampered both basic research and translational efforts. RESULTS: We report a detailed transcriptome and partial genome for E. gracilis Z1. The nuclear genome is estimated to be around 500 Mb in size, and the transcriptome encodes over 36,000 proteins and the genome possesses less than 1% coding sequence. Annotation of coding sequences indicates a highly sophisticated endomembrane system, RNA processing mechanisms and nuclear genome contributions from several photosynthetic lineages. Multiple gene families, including likely signal transduction components, have been massively expanded. Alterations in protein abundance are controlled post-transcriptionally between light and dark conditions, surprisingly similar to trypanosomatids. CONCLUSIONS: Our data provide evidence that a range of photosynthetic eukaryotes contributed to the Euglena nuclear genome, evidence in support of the 'shopping bag' hypothesis for plastid acquisition. We also suggest that euglenids possess unique regulatory mechanisms for achieving extreme adaptability, through mechanisms of paralog expansion and gene acquisition.

Biology Centre Institute of Parasitology Czech Academy of Sciences and Faculty of Sciences University of South Bohemia 37005 České Budějovice Czech Republic

Cell Biology Division Department of Biology University of Erlangen Nuremberg 91058 Erlangen Germany

Centro Andaluz de Biología del Desarrollo CSIC Pablo de Olavide University Seville Spain

Department of Biochemistry University of Cambridge Cambridge CB2 1QW UK

Department of Biological and Geographical Sciences School of Applied Sciences University of Huddersfield Queensgate Huddersfield HD1 3DH UK

Department of Biological and Medical Sciences Faculty of Health and Life Sciences Oxford Brookes University Oxford OX3 0BP UK

Department of Infection Biology Institute of Infection and Global Health University of Liverpool Liverpool UK

Department of Parasitology Faculty of Science Charles University BIOCEV 252 50 Vestec Czech Republic

Department of Plant Sciences University of Oxford Oxford OX1 3RB UK

Division of Infectious Disease Department of Medicine University of Alberta Edmonton Alberta T6G Canada

Division of Infectious Disease Department of Medicine University of Alberta Edmonton Alberta T6G Canada Department of Life Sciences The Natural History Museum Cromwell Road London SW7 5BD UK

Laboratory of Cellular and Structural Biology The Rockefeller University New York NY 10065 USA

School of Life Sciences University of Dundee Dundee DD1 5EH UK

School of Life Sciences University of Dundee Dundee DD1 5EH UK Biology Centre Institute of Parasitology Czech Academy of Sciences and Faculty of Sciences University of South Bohemia 37005 České Budějovice Czech Republic

School of Life Sciences University of Dundee Dundee DD1 5EH UK Department of Biochemistry University of Cambridge Cambridge CB2 1QW UK

Citace poskytuje Crossref.org

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