Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

Z. Kahounová, E. Slabáková, L. Binó, J. Remšík, R. Fedr, J. Bouchal, D. Kurfűrstová, R. Vrtěl, V. Študent, L. Jurečková, V. Porokh, A. Hampl, K. Souček,

. 2018 ; 33 (-) : 255-259. [pub] 20181116

Language English Country England, Great Britain

Document type Case Reports, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19034883

Grant support
NV15-28628A MZ0 CEP Register

A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5TM Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19034883
003      
CZ-PrNML
005      
20241217085412.0
007      
ta
008      
191007e20181116enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.scr.2018.11.006 $2 doi
035    __
$a (PubMed)30481741
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Kahounová, Zuzana $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
245    10
$a Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A / $c Z. Kahounová, E. Slabáková, L. Binó, J. Remšík, R. Fedr, J. Bouchal, D. Kurfűrstová, R. Vrtěl, V. Študent, L. Jurečková, V. Porokh, A. Hampl, K. Souček,
520    9_
$a A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5TM Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.
650    _2
$a senioři $7 D000368
650    _2
$a fibroblasty asociované s nádorem $x metabolismus $7 D000072645
650    _2
$a fibroblasty $x metabolismus $7 D005347
650    _2
$a lidé $7 D006801
650    _2
$a indukované pluripotentní kmenové buňky $x metabolismus $7 D057026
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a nádory prostaty $x genetika $7 D011471
655    _2
$a kazuistiky $7 D002363
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Slabáková, Eva $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.
700    1_
$a Binó, Lucia $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic. $7 xx0326742
700    1_
$a Remšík, Ján $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
700    1_
$a Fedr, Radek $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
700    1_
$a Bouchal, Jan $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
700    1_
$a Kurfürstová, Daniela $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic. $7 xx0269558
700    1_
$a Vrtěl, Radek $u Department of Medical Genetics, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
700    1_
$a Študent, Vladimír $u Department of Urology, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
700    1_
$a Jurečková, Lucie $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Porokh, Volodymyr $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Hampl, Aleš $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
700    1_
$a Souček, Karel $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. Electronic address: ksoucek@ibp.cz.
773    0_
$w MED00167579 $t Stem cell research $x 1876-7753 $g Roč. 33 (20181116), s. 255-259
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30481741 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20241217085408 $b ABA008
999    __
$a ok $b bmc $g 1451543 $s 1073433
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 33 $c - $d 255-259 $e 20181116 $i 1876-7753 $m Stem cell research $n Stem Cell Res $x MED00167579
GRA    __
$a NV15-28628A $p MZ0
LZP    __
$a Pubmed-20191007

Find record

Citation metrics

Archiving options