• Je něco špatně v tomto záznamu ?

Plastidial Phosphoglucose Isomerase Is an Important Determinant of Seed Yield through Its Involvement in Gibberellin-Mediated Reproductive Development and Storage Reserve Biosynthesis in Arabidopsis

A. Bahaji, G. Almagro, I. Ezquer, S. Gámez-Arcas, ÁM. Sánchez-López, FJ. Muñoz, RJ. Barrio, MC. Sampedro, N. De Diego, L. Spíchal, K. Doležal, D. Tarkowská, E. Caporali, MA. Mendes, E. Baroja-Fernández, J. Pozueta-Romero,

. 2018 ; 30 (9) : 2082-2098. [pub] 20180810

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19035079

The plastid-localized phosphoglucose isomerase isoform PGI1 is an important determinant of growth in Arabidopsis thaliana, likely due to its involvement in the biosynthesis of plastidial isoprenoid-derived hormones. Here, we investigated whether PGI1 also influences seed yields. PGI1 is strongly expressed in maturing seed embryos and vascular tissues. PGI1-null pgi1-2 plants had ∼60% lower seed yields than wild-type plants, with reduced numbers of inflorescences and thus fewer siliques and seeds per plant. These traits were associated with low bioactive gibberellin (GA) contents. Accordingly, wild-type phenotypes were restored by exogenous GA application. pgi1-2 seeds were lighter and accumulated ∼50% less fatty acids (FAs) and ∼35% less protein than wild-type seeds. Seeds of cytokinin-deficient plants overexpressing CYTOKININ OXIDASE/DEHYDROGENASE1 (35S:AtCKX1) and GA-deficient ga20ox1 ga20ox2 mutants did not accumulate low levels of FAs, and exogenous application of the cytokinin 6-benzylaminopurine and GAs did not rescue the reduced weight and FA content of pgi1-2 seeds. Seeds from reciprocal crosses between pgi1-2 and wild-type plants accumulated wild-type levels of FAs and proteins. Therefore, PGI1 is an important determinant of Arabidopsis seed yield due to its involvement in two processes: GA-mediated reproductive development and the metabolic conversion of plastidial glucose-6-phosphate to storage reserves in the embryo.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035079
003      
CZ-PrNML
005      
20191014120817.0
007      
ta
008      
191007s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1105/tpc.18.00312 $2 doi
035    __
$a (PubMed)30099384
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Bahaji, Abdellatif $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain.
245    10
$a Plastidial Phosphoglucose Isomerase Is an Important Determinant of Seed Yield through Its Involvement in Gibberellin-Mediated Reproductive Development and Storage Reserve Biosynthesis in Arabidopsis / $c A. Bahaji, G. Almagro, I. Ezquer, S. Gámez-Arcas, ÁM. Sánchez-López, FJ. Muñoz, RJ. Barrio, MC. Sampedro, N. De Diego, L. Spíchal, K. Doležal, D. Tarkowská, E. Caporali, MA. Mendes, E. Baroja-Fernández, J. Pozueta-Romero,
520    9_
$a The plastid-localized phosphoglucose isomerase isoform PGI1 is an important determinant of growth in Arabidopsis thaliana, likely due to its involvement in the biosynthesis of plastidial isoprenoid-derived hormones. Here, we investigated whether PGI1 also influences seed yields. PGI1 is strongly expressed in maturing seed embryos and vascular tissues. PGI1-null pgi1-2 plants had ∼60% lower seed yields than wild-type plants, with reduced numbers of inflorescences and thus fewer siliques and seeds per plant. These traits were associated with low bioactive gibberellin (GA) contents. Accordingly, wild-type phenotypes were restored by exogenous GA application. pgi1-2 seeds were lighter and accumulated ∼50% less fatty acids (FAs) and ∼35% less protein than wild-type seeds. Seeds of cytokinin-deficient plants overexpressing CYTOKININ OXIDASE/DEHYDROGENASE1 (35S:AtCKX1) and GA-deficient ga20ox1 ga20ox2 mutants did not accumulate low levels of FAs, and exogenous application of the cytokinin 6-benzylaminopurine and GAs did not rescue the reduced weight and FA content of pgi1-2 seeds. Seeds from reciprocal crosses between pgi1-2 and wild-type plants accumulated wild-type levels of FAs and proteins. Therefore, PGI1 is an important determinant of Arabidopsis seed yield due to its involvement in two processes: GA-mediated reproductive development and the metabolic conversion of plastidial glucose-6-phosphate to storage reserves in the embryo.
650    _2
$a Arabidopsis $x enzymologie $x metabolismus $7 D017360
650    _2
$a proteiny huseníčku $x genetika $x metabolismus $7 D029681
650    _2
$a regulace genové exprese u rostlin $7 D018506
650    _2
$a gibereliny $x metabolismus $7 D005875
650    _2
$a glukosa-6-fosfát $x metabolismus $7 D019298
650    _2
$a glukosa-6-fosfátisomerasa $x genetika $x metabolismus $7 D005956
650    _2
$a membránové proteiny $x genetika $x metabolismus $7 D008565
650    _2
$a oxidoreduktasy působící na CH-NH vazby $x genetika $x metabolismus $7 D000587
650    _2
$a plastidy $x metabolismus $7 D018087
650    _2
$a semena rostlinná $x enzymologie $x metabolismus $7 D012639
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Almagro, Goizeder $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain.
700    1_
$a Ezquer, Ignacio $u Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy.
700    1_
$a Gámez-Arcas, Samuel $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain.
700    1_
$a Sánchez-López, Ángela María $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain.
700    1_
$a Muñoz, Francisco José $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain.
700    1_
$a Barrio, Ramón José $u Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, UPV/EHU, E-01006 Vitoria-Gasteiz, Spain.
700    1_
$a Sampedro, M Carmen $u Central Service of Analysis of Alava, SGIker, University of the Basque Country, UPV/EHU, E-01006 Vitoria-Gasteiz, Spain.
700    1_
$a De Diego, Nuria $u Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, CZ-78371 Olomouc, Czech Republic.
700    1_
$a Spíchal, Lukáš $u Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, CZ-78371 Olomouc, Czech Republic.
700    1_
$a Doležal, Karel $u Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, CZ-78371 Olomouc, Czech Republic. Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR and Faculty of Science, Palacký University, CZ-78371 Olomouc, Czech Republic.
700    1_
$a Tarkowská, Danuše $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR and Faculty of Science, Palacký University, CZ-78371 Olomouc, Czech Republic.
700    1_
$a Caporali, Elisabetta $u Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy.
700    1_
$a Mendes, Marta Adelina $u Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy.
700    1_
$a Baroja-Fernández, Edurne $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain.
700    1_
$a Pozueta-Romero, Javier $u Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), 31192 Mutiloabeti, Nafarroa, Spain javier.pozueta@csic.es.
773    0_
$w MED00005315 $t The Plant cell $x 1532-298X $g Roč. 30, č. 9 (2018), s. 2082-2098
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30099384 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191014121242 $b ABA008
999    __
$a ok $b bmc $g 1451739 $s 1073629
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 30 $c 9 $d 2082-2098 $e 20180810 $i 1532-298X $m The Plant cell $n Plant Cell $x MED00005315
LZP    __
$a Pubmed-20191007

Najít záznam

Citační ukazatele

    Možnosti archivace