• Je něco špatně v tomto záznamu ?

The Sm-core mediates the retention of partially-assembled spliceosomal snRNPs in Cajal bodies until their full maturation

A. Roithová, K. Klimešová, J. Pánek, CL. Will, R. Lührmann, D. Stanek, C. Girard,

. 2018 ; 46 (7) : 3774-3790. [pub] 20180420

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19035410

Cajal bodies (CBs) are nuclear non-membrane bound organelles where small nuclear ribonucleoprotein particles (snRNPs) undergo their final maturation and quality control before they are released to the nucleoplasm. However, the molecular mechanism how immature snRNPs are targeted and retained in CBs has yet to be described. Here, we microinjected and expressed various snRNA deletion mutants as well as chimeric 7SK, Alu or bacterial SRP non-coding RNAs and provide evidence that Sm and SMN binding sites are necessary and sufficient for CB localization of snRNAs. We further show that Sm proteins, and specifically their GR-rich domains, are important for accumulating snRNPs in CBs. Accordingly, core snRNPs containing the Sm proteins, but not naked snRNAs, restore the formation of CBs after their depletion. Finally, we show that immature but not fully assembled snRNPs are able to induce CB formation and that microinjection of an excess of U2 snRNP-specific proteins, which promotes U2 snRNP maturation, chases U2 snRNA from CBs. We propose that the accessibility of the Sm ring represents the molecular basis for the quality control of the final maturation of snRNPs and the sequestration of immature particles in CBs.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19035410
003      
CZ-PrNML
005      
20191008113002.0
007      
ta
008      
191007s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/nar/gky070 $2 doi
035    __
$a (PubMed)29415178
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Roithová, Adriana $u Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
245    14
$a The Sm-core mediates the retention of partially-assembled spliceosomal snRNPs in Cajal bodies until their full maturation / $c A. Roithová, K. Klimešová, J. Pánek, CL. Will, R. Lührmann, D. Stanek, C. Girard,
520    9_
$a Cajal bodies (CBs) are nuclear non-membrane bound organelles where small nuclear ribonucleoprotein particles (snRNPs) undergo their final maturation and quality control before they are released to the nucleoplasm. However, the molecular mechanism how immature snRNPs are targeted and retained in CBs has yet to be described. Here, we microinjected and expressed various snRNA deletion mutants as well as chimeric 7SK, Alu or bacterial SRP non-coding RNAs and provide evidence that Sm and SMN binding sites are necessary and sufficient for CB localization of snRNAs. We further show that Sm proteins, and specifically their GR-rich domains, are important for accumulating snRNPs in CBs. Accordingly, core snRNPs containing the Sm proteins, but not naked snRNAs, restore the formation of CBs after their depletion. Finally, we show that immature but not fully assembled snRNPs are able to induce CB formation and that microinjection of an excess of U2 snRNP-specific proteins, which promotes U2 snRNP maturation, chases U2 snRNA from CBs. We propose that the accessibility of the Sm ring represents the molecular basis for the quality control of the final maturation of snRNPs and the sequestration of immature particles in CBs.
650    _2
$a buněčné jádro $x genetika $7 D002467
650    _2
$a Cajalova tělíska $x genetika $x metabolismus $7 D020541
650    _2
$a regulace genové exprese $x genetika $7 D005786
650    _2
$a HeLa buňky $7 D006367
650    _2
$a lidé $7 D006801
650    _2
$a RNA malá jaderná $x genetika $7 D012342
650    _2
$a malý jaderný ribonukleoprotein U2 $x genetika $7 D017413
650    _2
$a spliceozomy $x genetika $7 D017381
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Klimešová, Klára $u Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Pánek, Josef $u Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Will, Cindy L $u Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
700    1_
$a Lührmann, Reinhard $u Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
700    1_
$a Stanek, David $u Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Girard, Cyrille $u Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
773    0_
$w MED00003554 $t Nucleic acids research $x 1362-4962 $g Roč. 46, č. 7 (2018), s. 3774-3790
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29415178 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20191007 $b ABA008
991    __
$a 20191008113418 $b ABA008
999    __
$a ok $b bmc $g 1452070 $s 1073960
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 46 $c 7 $d 3774-3790 $e 20180420 $i 1362-4962 $m Nucleic acids research $n Nucleic Acids Res $x MED00003554
LZP    __
$a Pubmed-20191007

Najít záznam

Citační ukazatele

Možnosti archivace