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Excessive tubulin polyglutamylation causes neurodegeneration and perturbs neuronal transport

MM. Magiera, S. Bodakuntla, J. Žiak, S. Lacomme, P. Marques Sousa, S. Leboucher, TJ. Hausrat, C. Bosc, A. Andrieux, M. Kneussel, M. Landry, A. Calas, M. Balastik, C. Janke,

. 2018 ; 37 (23) : . [pub] 20181112

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045142

Posttranslational modifications of tubulin are emerging regulators of microtubule functions. We have shown earlier that upregulated polyglutamylation is linked to rapid degeneration of Purkinje cells in mice with a mutation in the deglutamylating enzyme CCP1. How polyglutamylation leads to degeneration, whether it affects multiple neuron types, or which physiological processes it regulates in healthy neurons has remained unknown. Here, we demonstrate that excessive polyglutamylation induces neurodegeneration in a cell-autonomous manner and can occur in many parts of the central nervous system. Degeneration of selected neurons in CCP1-deficient mice can be fully rescued by simultaneous knockout of the counteracting polyglutamylase TTLL1. Excessive polyglutamylation reduces the efficiency of neuronal transport in cultured hippocampal neurons, suggesting that impaired cargo transport plays an important role in the observed degenerative phenotypes. We thus establish polyglutamylation as a cell-autonomous mechanism for neurodegeneration that might be therapeutically accessible through manipulation of the enzymes that control this posttranslational modification.

Citace poskytuje Crossref.org

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$a Bodakuntla, Satish $u Institut Curie, CNRS UMR3348, PSL Research University, Orsay, France. Université Paris-Saclay, CNRS UMR3348, Université Paris Sud, Orsay, France.
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$a Žiak, Jakub $u Department of Molecular Neurobiology, Institute of Physiology, Czech Academy of Sciences, Prague 4, Czech Republic. Faculty of Science, Charles University, Prague 2, Czech Republic.
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$a Lacomme, Sabrina $u Bordeaux Imaging Center, BIC, UMS 3420, Université Bordeaux, Bordeaux, France.
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$a Marques Sousa, Patricia $u Institut Curie, CNRS UMR3348, PSL Research University, Orsay, France. Université Paris-Saclay, CNRS UMR3348, Université Paris Sud, Orsay, France.
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$a Leboucher, Sophie $u Institut Curie, CNRS UMR3348, PSL Research University, Orsay, France. Université Paris-Saclay, CNRS UMR3348, Université Paris Sud, Orsay, France.
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$a Hausrat, Torben J $u Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
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$a Bosc, Christophe $u Grenoble Institut des Neurosciences, GIN, Université Grenoble Alpes, Grenoble, France. Inserm U1216, Grenoble, France.
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$a Andrieux, Annie $u Grenoble Institut des Neurosciences, GIN, Université Grenoble Alpes, Grenoble, France. Inserm U1216, Grenoble, France.
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