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Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation
G. Dayanithi, O. Forostyak, S. Forostyak, T. Kayano, Y. Ueta, A. Verkhratsky,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
Nature Open Access
od 2011-12-01
PubMed Central
od 2011
Europe PubMed Central
od 2011
ProQuest Central
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Health & Medicine (ProQuest)
od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
Springer Nature OA/Free Journals
od 2011-12-01
- MeSH
- dehydratace metabolismus MeSH
- exocytóza * MeSH
- fluorescence MeSH
- laktace * MeSH
- nervové receptory metabolismus MeSH
- nocicepce MeSH
- oxytocin metabolismus MeSH
- potkani transgenní MeSH
- receptory oxytocinu metabolismus MeSH
- receptory vasopresinů metabolismus MeSH
- spinální ganglia metabolismus MeSH
- vasopresiny metabolismus MeSH
- zadní lalok hypofýzy metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The neurohormones arginine-vasopressin (AVP) and oxytocin (OT) synthesised in supraoptic and paraventricular nuclei of neurohypophysis regulate lactation, systemic water homeostasis and nociception. Using transgenic rats expressing AVP and OT tagged with fluorescent proteins we demonstrate that both neurohormones are expressed in sensory neurones both in vitro, in primary cultures, and in situ, in the intact ganglia; this expression was further confirmed with immunocytochemistry. Both neurohormones were expressed in nociceptive neurones immunopositive to transient receptor potential vannilloid 1 (TRPV1) channel antibodies. The AVP and OT-expressing DRG neurones responded to AVP, OT, 50 mM K+ and capsaicin with [Ca2+]i transients; responses to AVP and OT were specifically blocked by the antagonists of V1 AVP and OT receptors. Probing the extracellular incubation saline with ELISA revealed AVP and OT secretion from isolated DRGs; this secretion was inhibited by tetanus toxin (TeNT) indicating the role for vesicular release. Expression of OT, but not AVP in DRG neurones significantly increased during lactation. Together, the results indicate novel physiological roles (possibly related to nociception and mood regulation) of AVP and OT in the sensory neurones.
Citace poskytuje Crossref.org
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- $a Dayanithi, Govindan $u Institut des Sciences Biologiques-Neurosciences, cognition, Centre Nationale de la Recherche Scientifique, 3 rue Michel-Ange, 75794, Paris cedex 16, France. gdaya@univ-montp2.fr. MMDN-Institut National de la Santé et de la Recherche Médicale-U1198, Université de Montpellier, 34095, Montpellier, France. gdaya@univ-montp2.fr. Ecole Pratique des Hautes Etudes, Sorbonne, Les Patios Saint-Jaques, 75014, Paris, France. gdaya@univ-montp2.fr. Department of Pharmacology and Toxicology, Faculty of Medicine, Charles University at Plzen, CZ-32300, Plzen, Czech Republic. gdaya@univ-montp2.fr.
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- $a Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation / $c G. Dayanithi, O. Forostyak, S. Forostyak, T. Kayano, Y. Ueta, A. Verkhratsky,
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- $a The neurohormones arginine-vasopressin (AVP) and oxytocin (OT) synthesised in supraoptic and paraventricular nuclei of neurohypophysis regulate lactation, systemic water homeostasis and nociception. Using transgenic rats expressing AVP and OT tagged with fluorescent proteins we demonstrate that both neurohormones are expressed in sensory neurones both in vitro, in primary cultures, and in situ, in the intact ganglia; this expression was further confirmed with immunocytochemistry. Both neurohormones were expressed in nociceptive neurones immunopositive to transient receptor potential vannilloid 1 (TRPV1) channel antibodies. The AVP and OT-expressing DRG neurones responded to AVP, OT, 50 mM K+ and capsaicin with [Ca2+]i transients; responses to AVP and OT were specifically blocked by the antagonists of V1 AVP and OT receptors. Probing the extracellular incubation saline with ELISA revealed AVP and OT secretion from isolated DRGs; this secretion was inhibited by tetanus toxin (TeNT) indicating the role for vesicular release. Expression of OT, but not AVP in DRG neurones significantly increased during lactation. Together, the results indicate novel physiological roles (possibly related to nociception and mood regulation) of AVP and OT in the sensory neurones.
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- $a Forostyak, Oksana $u Department of Molecular Neurophysiology, Institute of Experimental Medicine, Czech Academy of Sciences, 14220, Prague, Czech Republic.
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- $a Kayano, Tomohiko $u MMDN-Institut National de la Santé et de la Recherche Médicale-U1198, Université de Montpellier, 34095, Montpellier, France. Ecole Pratique des Hautes Etudes, Sorbonne, Les Patios Saint-Jaques, 75014, Paris, France. Department of Molecular Neurophysiology, Institute of Experimental Medicine, Czech Academy of Sciences, 14220, Prague, Czech Republic.
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- $a Ueta, Yoichi $u Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, 807-8555, Japan.
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- $a Verkhratsky, Alexei $u Faculty of Biology, Medicine and Health, University of Manchester, M13 9PT, Manchester, UK. Alexej.Verkhratsky@manchester.ac.uk. Achucarro Centre for Neuroscience, IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain. Alexej.Verkhratsky@manchester.ac.uk.
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