The neurohormones arginine-vasopressin (AVP) and oxytocin (OT) synthesised in supraoptic and paraventricular nuclei of neurohypophysis regulate lactation, systemic water homeostasis and nociception. Using transgenic rats expressing AVP and OT tagged with fluorescent proteins we demonstrate that both neurohormones are expressed in sensory neurones both in vitro, in primary cultures, and in situ, in the intact ganglia; this expression was further confirmed with immunocytochemistry. Both neurohormones were expressed in nociceptive neurones immunopositive to transient receptor potential vannilloid 1 (TRPV1) channel antibodies. The AVP and OT-expressing DRG neurones responded to AVP, OT, 50 mM K+ and capsaicin with [Ca2+]i transients; responses to AVP and OT were specifically blocked by the antagonists of V1 AVP and OT receptors. Probing the extracellular incubation saline with ELISA revealed AVP and OT secretion from isolated DRGs; this secretion was inhibited by tetanus toxin (TeNT) indicating the role for vesicular release. Expression of OT, but not AVP in DRG neurones significantly increased during lactation. Together, the results indicate novel physiological roles (possibly related to nociception and mood regulation) of AVP and OT in the sensory neurones.
- MeSH
- dehydratace metabolismus MeSH
- exocytóza * MeSH
- fluorescence MeSH
- laktace * MeSH
- nervové receptory metabolismus MeSH
- nocicepce MeSH
- oxytocin metabolismus MeSH
- potkani transgenní MeSH
- receptory oxytocinu metabolismus MeSH
- receptory vasopresinů metabolismus MeSH
- spinální ganglia metabolismus MeSH
- vasopresiny metabolismus MeSH
- zadní lalok hypofýzy metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Statins have revolutionized the treatment of hypercholesterolemia due to their ability to inhibit cholesterol biosynthesis. Their immunomodulatory and anti-inflammatory effects and positive effects on the treatment of atherosclerosis and its complications are well known. Here, we describe the effects of statins on the treatment of presbycusis in C57BL/6J mice. In this strain with accelerated aging, we demonstrate that animals treated with atorvastatin (10mg/kg per day in chow diet) for 2 months showed larger amplitudes of distortion product otoacoustic emissions (DPOAE) than did the non-treated control group. This finding indicates a better survival of outer hair cell function in the inner ear of C57BL/6J mice. The observed decreased expression of intercellular and vascular adhesion molecules in the aortic wall of atorvastatin-treated animals suggests that reducing endothelial inflammatory effects may contribute to the positive effect of atorvastatin on the amplitudes of DPOAE by influencing the blood supply to the inner ear. No such beneficial effect of statins was found in apoE(-/-) mice treated with atorvastatin under the same conditions. Our results suggest that statins could also slow down the age-related deterioration of hearing in man.
- MeSH
- akustická stimulace MeSH
- apolipoproteiny E nedostatek MeSH
- časové faktory MeSH
- exprese genu genetika MeSH
- financování organizované MeSH
- kyseliny heptylové terapeutické užití MeSH
- mezibuněčná adhezivní molekula-1 metabolismus MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- otoakustické emise spontánní účinky léků MeSH
- potrava speciální MeSH
- presbyakuze dietoterapie patologie prevence a kontrola MeSH
- pyrroly terapeutické užití MeSH
- receptory vasopresinů metabolismus MeSH
- sluchový práh fyziologie účinky léků MeSH
- stárnutí MeSH
- statiny terapeutické užití MeSH
- vnitřní ucho patofyziologie MeSH
- vztah dávky záření a odpovědi MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
