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Stress response of Escherichia coli to essential oil components - insights on low-molecular-weight proteins from MALDI-TOF

M. Božik, P. Cejnar, M. Šašková, P. Nový, P. Maršík, P. Klouček,

. 2018 ; 8 (1) : 13042. [pub] 20180829

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045270

The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.

Citace poskytuje Crossref.org

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$a Cejnar, Pavel $u University of Chemistry and Technology, Department of Computing and Control Engineering, Prague, Czech Republic. Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources, Department of Plant Protection, Prague, Czech Republic.
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$a Šašková, Martina $u Czech University of Life Sciences, Faculty of Agrobiology, Food and Natural Resources, Department of Quality of Agricultural Products, Prague, Czech Republic.
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