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An integrated single cell and spatial transcriptomic map of human white adipose tissue
L. Massier, J. Jalkanen, M. Elmastas, J. Zhong, T. Wang, PA. Nono Nankam, S. Frendo-Cumbo, J. Bäckdahl, N. Subramanian, T. Sekine, AG. Kerr, BTP. Tseng, J. Laurencikiene, M. Buggert, M. Lourda, K. Kublickiene, N. Bhalla, A. Andersson, A....
Language English Country England, Great Britain
Document type Meta-Analysis, Journal Article
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- MeSH
- Adipogenesis genetics MeSH
- Adipose Tissue, White * metabolism MeSH
- Humans MeSH
- Gene Expression Profiling MeSH
- Transcriptome * genetics MeSH
- Adipose Tissue MeSH
- Adipocytes metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Meta-Analysis MeSH
To date, single-cell studies of human white adipose tissue (WAT) have been based on small cohort sizes and no cellular consensus nomenclature exists. Herein, we performed a comprehensive meta-analysis of publicly available and newly generated single-cell, single-nucleus, and spatial transcriptomic results from human subcutaneous, omental, and perivascular WAT. Our high-resolution map is built on data from ten studies and allowed us to robustly identify >60 subpopulations of adipocytes, fibroblast and adipogenic progenitors, vascular, and immune cells. Using these results, we deconvolved spatial and bulk transcriptomic data from nine additional cohorts to provide spatial and clinical dimensions to the map. This identified cell-cell interactions as well as relationships between specific cell subtypes and insulin resistance, dyslipidemia, adipocyte volume, and lipolysis upon long-term weight changes. Altogether, our meta-map provides a rich resource defining the cellular and microarchitectural landscape of human WAT and describes the associations between specific cell types and metabolic states.
Department of Obesity and Nutritional Sciences The Novo Nordisk Foundation Hellerup Denmark
Institut Universitaire de France Paris France
Institute of Metabolic and Cardiovascular Diseases Université de Toulouse UMR1297 Toulouse France
Laboratoire de biochimie Centre Hospitalier Universitaire de Toulouse Toulouse France
References provided by Crossref.org
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