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Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration

MGMC. Mori da Cunha, G. Giacomazzi, G. Callewaert, L. Hympanova, F. Russo, G. Vande Velde, R. Gijsbers, M. Albersen, M. Sampaolesi, J. Deprest,

. 2018 ; 8 (1) : 10604. [pub] 20180713

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19045370

Currently cell therapy is considered as an experimental strategy to assist the healing process following simulated vaginal birth injury in rats, boosting the functional and morphologic recovery of pelvic floor muscles and nerves. However, the optimal administration route and dose still need to be determined. Mesangioblasts theoretically have the advantage that they can differentiate in skeletal and smooth muscle. We investigated the fate of mesoangioblasts transduced with luciferase and green fluorescent protein reporter genes (rMABseGFP/fLUC) using bioluminescence, immunofluorescence and RT-PCR in rats undergoing simulated birth injury. rMABseGFP/fLUC were injected locally, intravenously and intra-arterially (common iliacs and aorta). Intra-arterial delivery resulted in the highest amount of rMABseGFP/fLUC in the pelvic organs region and in a more homogeneous distribution over all relevant pelvic organs. Sham controls showed that the presence of the injury is important for recruitment of intra-arterially injected rMABseGFP/fLUC. Injection through the aorta or bilaterally in the common iliac arteries resulted in comparable numbers of rMABseGFP/fLUC in the pelvic organs, yet aortic injection was faster and gave less complications.

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$a Mori da Cunha, Marina Gabriela Monteiro Carvalho $u Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium. Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
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$a Currently cell therapy is considered as an experimental strategy to assist the healing process following simulated vaginal birth injury in rats, boosting the functional and morphologic recovery of pelvic floor muscles and nerves. However, the optimal administration route and dose still need to be determined. Mesangioblasts theoretically have the advantage that they can differentiate in skeletal and smooth muscle. We investigated the fate of mesoangioblasts transduced with luciferase and green fluorescent protein reporter genes (rMABseGFP/fLUC) using bioluminescence, immunofluorescence and RT-PCR in rats undergoing simulated birth injury. rMABseGFP/fLUC were injected locally, intravenously and intra-arterially (common iliacs and aorta). Intra-arterial delivery resulted in the highest amount of rMABseGFP/fLUC in the pelvic organs region and in a more homogeneous distribution over all relevant pelvic organs. Sham controls showed that the presence of the injury is important for recruitment of intra-arterially injected rMABseGFP/fLUC. Injection through the aorta or bilaterally in the common iliac arteries resulted in comparable numbers of rMABseGFP/fLUC in the pelvic organs, yet aortic injection was faster and gave less complications.
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$a Hympanova, Lucie $u Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium. Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium. Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
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$a Russo, Francesca $u Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium. Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
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$a Vande Velde, Greetje $u Molecular Small Animal Imaging Center, KU Leuven, 3000, Leuven, Belgium.
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$a Gijsbers, Rik $u Laboratory for Molecular Virology and Gene Therapy, KU Leuven, Flanders, Belgium.
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$a Albersen, Maarten $u Department of Urology, University Hospitals Leuven, Leuven, Belgium.
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$a Sampaolesi, Maurilio $u Translational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, Leuven, Belgium.
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$a Deprest, Jan $u Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium. jan.deprest@uzleuven.be. Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium. jan.deprest@uzleuven.be. Pelvic Floor Unit, University Hospitals KU Leuven, Leuven, Belgium. jan.deprest@uzleuven.be.
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