Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer

J. Juracek, B. Peltanova, J. Dolezel, M. Fedorko, D. Pacik, L. Radova, P. Vesela, M. Svoboda, O. Slaby, M. Stanik,

. 2018 ; 22 (3) : 2033-2038. [pub] 20180124

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19045562

Grantová podpora
NV15-31071A MZ0 CEP - Centrální evidence projektů

Urinary microRNAs (miRNAs) are emerging as clinically useful tool for early and non-invasive detection of various types of cancer including bladder cancer (BCA). In this study, 205 patients with BCA and 99 healthy controls were prospectively enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays (2578 miRNAs) and candidate miRNAs further validated in independent cohorts using qRT-PCR. Whole-genome profiling identified 76 miRNAs with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the training and independent validation phase of the study, miR-31-5p, miR-93-5p and miR-191-5p were confirmed to have significantly higher levels in urine of patients with BCA in comparison with controls (P < 0.01). We further established 2-miRNA-based urinary DxScore (miR-93-5p, miR-31-5p) enabling sensitive BCA detection with AUC being 0.84 and 0.81 in the training and validation phase, respectively. Moreover, DxScore significantly differed in the various histopathological subgroups of BCA and decreased post-operatively. In conclusion, we identified and independently validated cell-free urinary miRNAs as promising biomarkers enabling non-invasive detection of BCA.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19045562
003      
CZ-PrNML
005      
20201104115642.0
007      
ta
008      
200109s2018 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1111/jcmm.13487 $2 doi
035    __
$a (PubMed)29363887
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Juracek, Jaroslav $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
245    10
$a Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer / $c J. Juracek, B. Peltanova, J. Dolezel, M. Fedorko, D. Pacik, L. Radova, P. Vesela, M. Svoboda, O. Slaby, M. Stanik,
520    9_
$a Urinary microRNAs (miRNAs) are emerging as clinically useful tool for early and non-invasive detection of various types of cancer including bladder cancer (BCA). In this study, 205 patients with BCA and 99 healthy controls were prospectively enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays (2578 miRNAs) and candidate miRNAs further validated in independent cohorts using qRT-PCR. Whole-genome profiling identified 76 miRNAs with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the training and independent validation phase of the study, miR-31-5p, miR-93-5p and miR-191-5p were confirmed to have significantly higher levels in urine of patients with BCA in comparison with controls (P < 0.01). We further established 2-miRNA-based urinary DxScore (miR-93-5p, miR-31-5p) enabling sensitive BCA detection with AUC being 0.84 and 0.81 in the training and validation phase, respectively. Moreover, DxScore significantly differed in the various histopathological subgroups of BCA and decreased post-operatively. In conclusion, we identified and independently validated cell-free urinary miRNAs as promising biomarkers enabling non-invasive detection of BCA.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a nádorové biomarkery $x genetika $x moč $7 D014408
650    _2
$a karcinom $x diagnóza $x genetika $x patologie $x moč $7 D002277
650    _2
$a studie případů a kontrol $7 D016022
650    _2
$a ženské pohlaví $7 D005260
650    12
$a regulace genové exprese u nádorů $7 D015972
650    _2
$a genom lidský $7 D015894
650    _2
$a celogenomová asociační studie $7 D055106
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a mikro RNA $x genetika $x moč $7 D035683
650    _2
$a lidé středního věku $7 D008875
650    _2
$a stupeň nádoru $7 D060787
650    _2
$a prospektivní studie $7 D011446
650    _2
$a nádory močového měchýře $x diagnóza $x genetika $x patologie $x moč $7 D001749
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Peltanova, Barbora $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
700    1_
$a Dolezel, Jan $u Department of Urologic Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
700    1_
$a Fedorko, Michal $u Department of Urology, University Hospital Brno, Masaryk University Brno, Brno, Czech Republic.
700    1_
$a Pacik, Dalibor $u Department of Urology, University Hospital Brno, Masaryk University Brno, Brno, Czech Republic.
700    1_
$a Radova, Lenka $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
700    1_
$a Vesela, Petra $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
700    1_
$a Svoboda, Marek $u Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
700    1_
$a Slaby, Ondrej $u Central European Institute of Technology, Masaryk University, Brno, Czech Republic. Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
700    1_
$a Stanik, Michal $u Department of Urologic Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
773    0_
$w MED00006785 $t Journal of cellular and molecular medicine $x 1582-4934 $g Roč. 22, č. 3 (2018), s. 2033-2038
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29363887 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200109 $b ABA008
991    __
$a 20201104115640 $b ABA008
999    __
$a ok $b bmc $g 1483830 $s 1084235
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 22 $c 3 $d 2033-2038 $e 20180124 $i 1582-4934 $m Journal of cellular and molecular medicine $n J Cell Mol Med $x MED00006785
GRA    __
$a NV15-31071A $p MZ0
LZP    __
$a Pubmed-20200109

Najít záznam

Citační ukazatele

Možnosti archivace