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Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells
MT. Skowronski, P. Mlotkowska, D. Tanski, E. Lepiarczyk, B. Kempisty, L. Jaskiewicz, CS. Pareek, A. Skowronska,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
NLK
Free Medical Journals
od 2000
Freely Accessible Science Journals
od 2000
PubMed Central
od 2007
Europe PubMed Central
od 2007
ProQuest Central
od 2000-03-01
Open Access Digital Library
od 2000-01-01
Open Access Digital Library
od 2007-01-01
Health & Medicine (ProQuest)
od 2000-03-01
ROAD: Directory of Open Access Scholarly Resources
od 2000
PubMed
31623386
DOI
10.3390/ijms20194914
Knihovny.cz E-zdroje
- MeSH
- akvaporin 5 genetika MeSH
- biologické markery MeSH
- folikulární buňky účinky léků metabolismus MeSH
- folikuly stimulující hormon metabolismus MeSH
- hypofyzární hormony metabolismus farmakologie MeSH
- kokultivační techniky MeSH
- luteinizační hormon metabolismus MeSH
- ovariální folikul cytologie účinky léků metabolismus MeSH
- prasata MeSH
- prolaktin metabolismus MeSH
- regulace genové exprese u rostlin * MeSH
- růstový hormon metabolismus MeSH
- thekální buňky účinky léků metabolismus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
This study aimed to examine the effect of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) on Aquaporin 5 (AQP5) expression in granulosa (Gc) and theca cells (Tc) from medium (MF) and large (LF) ovarian follicles of pigs. The results showed that GH significantly decreased the expression of AQP5 in Gc from MF in relation to the control. In the Gc of large follicles, PRL stimulated the expression of AQP5. However, the increased expression of AQP5 in the Tc of LF was indicated by GH and PRL in relation to the control. A significantly higher expression of the AQP5 protein in the Gc from MF and LF was indicated by FSH and PRL. In co-cultures, an increased expression of AQP5 was observed in the Gc from LF incubated with LH, PRL, and GH. A significantly increased expression of AQP5 was also observed in co-cultures of Tc from all type of follicles incubated with LH, whereas PRL stimulated the expression of AQP5 in Tc from MF. Moreover, AQP5 protein expression increased in the co-culture isolated from MF and LF after treatment with FSH, LH, PRL, and GH. AQP5 immunoreactivity was observed in the cytoplasm, mainly in the perinuclear region and endosomes, as well as in the cell membranes of Gc and Tc from the LF and MF.
Department of Histology and Embryology
Veterinary Center Nicolaus Copernicus University in Torun 87 100 Torun Poland
Citace poskytuje Crossref.org
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- $a Skowronski, Mariusz T $u Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland. skowron@umk.pl.
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- $a Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells / $c MT. Skowronski, P. Mlotkowska, D. Tanski, E. Lepiarczyk, B. Kempisty, L. Jaskiewicz, CS. Pareek, A. Skowronska,
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- $a This study aimed to examine the effect of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) on Aquaporin 5 (AQP5) expression in granulosa (Gc) and theca cells (Tc) from medium (MF) and large (LF) ovarian follicles of pigs. The results showed that GH significantly decreased the expression of AQP5 in Gc from MF in relation to the control. In the Gc of large follicles, PRL stimulated the expression of AQP5. However, the increased expression of AQP5 in the Tc of LF was indicated by GH and PRL in relation to the control. A significantly higher expression of the AQP5 protein in the Gc from MF and LF was indicated by FSH and PRL. In co-cultures, an increased expression of AQP5 was observed in the Gc from LF incubated with LH, PRL, and GH. A significantly increased expression of AQP5 was also observed in co-cultures of Tc from all type of follicles incubated with LH, whereas PRL stimulated the expression of AQP5 in Tc from MF. Moreover, AQP5 protein expression increased in the co-culture isolated from MF and LF after treatment with FSH, LH, PRL, and GH. AQP5 immunoreactivity was observed in the cytoplasm, mainly in the perinuclear region and endosomes, as well as in the cell membranes of Gc and Tc from the LF and MF.
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- $a Kempisty, Bartosz $u Department of Histology and Embryology; Poznan University of Medical Sciences, 61-701 Poznan, Poland. bkempisty@ump.edu.pl. Department of Anatomy, Poznan University of Medical Sciences, 61-701 Poznan, Poland. bkempisty@ump.edu.pl. Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 602 00 Brno, Czech Republic. bkempisty@ump.edu.pl.
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- $a Skowronska, Agnieszka $u Department of Human Physiology, School of Medicine, University of Warmia and Mazury in Olsztyn, 10-752 Olsztyn, Poland. agnieszka.skowronska@uwm.edu.pl.
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