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Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom
SR. Smith, CL. Dupont, JK. McCarthy, JT. Broddrick, M. Oborník, A. Horák, Z. Füssy, J. Cihlář, S. Kleessen, H. Zheng, JP. McCrow, KK. Hixson, WL. Araújo, A. Nunes-Nesi, A. Fernie, Z. Nikoloski, BO. Palsson, AE. Allen,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
Free Medical Journals od 2010
Nature Open Access od 2010-12-01
PubMed Central od 2012
Europe PubMed Central od 2012
ProQuest Central od 2010-01-01
Open Access Digital Library od 2015-01-01
Open Access Digital Library od 2015-01-01
Medline Complete (EBSCOhost) od 2012-11-01
Health & Medicine (ProQuest) od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources od 2010
Odkazy
PubMed
31591397
DOI
10.1038/s41467-019-12407-y
Knihovny.cz E-zdroje
- MeSH
- biologické modely MeSH
- chloroplasty genetika metabolismus MeSH
- dusičnany metabolismus MeSH
- dusík metabolismus MeSH
- metabolické sítě a dráhy genetika MeSH
- metabolomika metody MeSH
- mitochondrie genetika metabolismus MeSH
- molekulární evoluce MeSH
- mořská voda mikrobiologie MeSH
- proteomika metody MeSH
- regulace genové exprese MeSH
- rozsivky genetika metabolismus MeSH
- signální transdukce genetika MeSH
- stanovení celkové genové exprese metody MeSH
- uhlík metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Diatoms outcompete other phytoplankton for nitrate, yet little is known about the mechanisms underpinning this ability. Genomes and genome-enabled studies have shown that diatoms possess unique features of nitrogen metabolism however, the implications for nutrient utilization and growth are poorly understood. Using a combination of transcriptomics, proteomics, metabolomics, fluxomics, and flux balance analysis to examine short-term shifts in nitrogen utilization in the model pennate diatom in Phaeodactylum tricornutum, we obtained a systems-level understanding of assimilation and intracellular distribution of nitrogen. Chloroplasts and mitochondria are energetically integrated at the critical intersection of carbon and nitrogen metabolism in diatoms. Pathways involved in this integration are organelle-localized GS-GOGAT cycles, aspartate and alanine systems for amino moiety exchange, and a split-organelle arginine biosynthesis pathway that clarifies the role of the diatom urea cycle. This unique configuration allows diatoms to efficiently adjust to changing nitrogen status, conferring an ecological advantage over other phytoplankton taxa.
Departamento de Biologia Vegetal Universidade Federal de Viçosa Viçosa Minas Gerais 36570 900 Brazil
Department of Bioengineering University of California San Diego La Jolla CA 92093 USA
Max Planck Institut of Molecular Plant Physiology Am Mühlenberg 1 14476 Potsdam Germany
Microbial and Environmental Genomics J Craig Venter Institute La Jolla CA 92037 USA
Targenomix GmbH Wissenschaftspark Potsdam Golm 14476 Potsdam Germany
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