• Something wrong with this record ?

Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells

Z. Skubalova, H. Michalkova, P. Michalek, V. Strmiska, R. Guran, MA. Merlos Rodrigo, K. Castkova, D. Hynek, V. Pekarik, O. Zitka, V. Adam, Z. Heger,

. 2019 ; 182 (-) : 110391. [pub] 20190724

Language English Country Netherlands

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc20006075

Nanoparticular form of titanium dioxide (TiO2 NPs) belongs to important industrial material. Despite being widely used, serious contradictions regarding biosafety of TiO2 NPs remain. We anticipate that such discrepancies could be due to a lack of understanding of a linkage between TiO2 NPs phase composition and cytotoxicity. Therefore, we synthesized two types of biphasic TiO2 NPs differing in an anatase-brookite phase composition. The study presents an array of in vitro data suggesting that TiO2 NPs with a prevailing anatase phase composition possess higher cytotoxicity compared to TiO2 NPs with an equal anatase-brookite crystallinity. This phenomenon was evidenced by significantly higher inhibition of metabolic activity and growth of epithelial and neuroblast-like cells. Moreover, anatase-prevailing TiO2 NPs tend to produce higher amount of reactive oxygen species resulting in DNA fragmentation. Further insights into the molecular aspects of cytotoxicity of anatase-prevailing TiO2 NPs were obtained by comparative proteomics delineating that TiO2 NPs deregulate expression of a variety of proteins and associated pathways. This inevitably results in a decreased cellular ability to detoxify reactive oxygen species and respond to various stress conditions. The study provides novel data that add another piece to the jigsaw of the relation between structural features of NPs and biosafety.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20006075
003      
CZ-PrNML
005      
20200527083548.0
007      
ta
008      
200511s2019 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.colsurfb.2019.110391 $2 doi
035    __
$a (PubMed)31377608
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Skubalova, Zuzana $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
245    10
$a Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells / $c Z. Skubalova, H. Michalkova, P. Michalek, V. Strmiska, R. Guran, MA. Merlos Rodrigo, K. Castkova, D. Hynek, V. Pekarik, O. Zitka, V. Adam, Z. Heger,
520    9_
$a Nanoparticular form of titanium dioxide (TiO2 NPs) belongs to important industrial material. Despite being widely used, serious contradictions regarding biosafety of TiO2 NPs remain. We anticipate that such discrepancies could be due to a lack of understanding of a linkage between TiO2 NPs phase composition and cytotoxicity. Therefore, we synthesized two types of biphasic TiO2 NPs differing in an anatase-brookite phase composition. The study presents an array of in vitro data suggesting that TiO2 NPs with a prevailing anatase phase composition possess higher cytotoxicity compared to TiO2 NPs with an equal anatase-brookite crystallinity. This phenomenon was evidenced by significantly higher inhibition of metabolic activity and growth of epithelial and neuroblast-like cells. Moreover, anatase-prevailing TiO2 NPs tend to produce higher amount of reactive oxygen species resulting in DNA fragmentation. Further insights into the molecular aspects of cytotoxicity of anatase-prevailing TiO2 NPs were obtained by comparative proteomics delineating that TiO2 NPs deregulate expression of a variety of proteins and associated pathways. This inevitably results in a decreased cellular ability to detoxify reactive oxygen species and respond to various stress conditions. The study provides novel data that add another piece to the jigsaw of the relation between structural features of NPs and biosafety.
650    _2
$a buněčné linie $7 D002460
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a krystalografie rentgenová $7 D018360
650    _2
$a epitelové buňky $x účinky léků $x metabolismus $7 D004847
650    _2
$a lidé $7 D006801
650    _2
$a kovové nanočástice $x chemie $x toxicita $x ultrastruktura $7 D053768
650    _2
$a transmisní elektronová mikroskopie $7 D046529
650    12
$a oxidační stres $7 D018384
650    _2
$a reaktivní formy kyslíku $x metabolismus $7 D017382
650    _2
$a titan $x chemie $x toxicita $7 D014025
655    _2
$a časopisecké články $7 D016428
700    1_
$a Michalkova, Hana $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
700    1_
$a Michalek, Petr $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Strmiska, Vladislav $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
700    1_
$a Guran, Roman $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Merlos Rodrigo, Miguel Angel $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Castkova, Klara $u Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Hynek, David $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Pekarik, Vladimir $u Institute of Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic.
700    1_
$a Zitka, Ondrej $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Adam, Vojtech $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic.
700    1_
$a Heger, Zbynek $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic. Electronic address: heger@mendelu.cz.
773    0_
$w MED00180202 $t Colloids and surfaces. B, Biointerfaces $x 1873-4367 $g Roč. 182, č. - (2019), s. 110391
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31377608 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20200527083545 $b ABA008
999    __
$a ok $b bmc $g 1524933 $s 1096131
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 182 $c - $d 110391 $e 20190724 $i 1873-4367 $m Colloids and surfaces. B, Biointerfaces $n Colloids surf., B Biointerfaces $x MED00180202
LZP    __
$a Pubmed-20200511

Find record

Citation metrics

Archiving options