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Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics
P. Klíma, V. Čermák, M. Srba, K. Müller, J. Petrášek, J. Šonka, L. Fischer, Z. Opatrný,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arabidopsis cytology genetics MeSH
- Cell Culture Techniques methods MeSH
- Cell Line MeSH
- Plants, Genetically Modified cytology genetics MeSH
- Genotyping Techniques methods MeSH
- Cloning, Molecular methods MeSH
- Flow Cytometry methods MeSH
- Gene Expression Profiling methods MeSH
- Nicotiana cytology genetics MeSH
- Transformation, Genetic MeSH
- Transcriptome MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Here we provide an overview of procedures for long-term cultivation, phenotyping, genotyping, and genetic transformation of cell cultures of tobacco cell lines BY-2 and VBI-0, and of A. thaliana, ecotype Landsberg erecta (LE) cell line. Notably, we present an improved protocol for BY-2 transformation and cloning and extend the available plant cell lines methodology toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Botany of the Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
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