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Essential Methods of Plant Sample Preparation for Light Microscopy
A. Soukup, E. Tylová,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Staining and Labeling methods MeSH
- Tissue Fixation methods MeSH
- Histocytochemistry methods MeSH
- Histocytological Preparation Techniques methods MeSH
- Microscopy methods MeSH
- Microtomy methods MeSH
- Plants chemistry ultrastructure MeSH
- Paraffin Embedding methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
There are various preparatory techniques for light microscopy permitting access to the inner structure of plant body and its development. Minute objects might be processed as whole-mount preparations, while voluminous ones should be separated into smaller pieces. Here we summarize some of the "classical" techniques to cut more voluminous objects into slices and access their inner structure either for simple anatomical analysis or for further processing (e.g., histochemistry, immunohistochemistry, in situ hybridization, enzyme histochemistry).
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
References provided by Crossref.org
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- $a There are various preparatory techniques for light microscopy permitting access to the inner structure of plant body and its development. Minute objects might be processed as whole-mount preparations, while voluminous ones should be separated into smaller pieces. Here we summarize some of the "classical" techniques to cut more voluminous objects into slices and access their inner structure either for simple anatomical analysis or for further processing (e.g., histochemistry, immunohistochemistry, in situ hybridization, enzyme histochemistry).
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