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Essential Methods of Plant Sample Preparation for Light Microscopy
A. Soukup, E. Tylová,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- barvení a značení metody MeSH
- fixace tkání metody MeSH
- histocytochemie metody MeSH
- histocytologické preparační techniky metody MeSH
- mikroskopie metody MeSH
- mikrotomie metody MeSH
- rostliny chemie ultrastruktura MeSH
- zalévání tkání do parafínu metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
There are various preparatory techniques for light microscopy permitting access to the inner structure of plant body and its development. Minute objects might be processed as whole-mount preparations, while voluminous ones should be separated into smaller pieces. Here we summarize some of the "classical" techniques to cut more voluminous objects into slices and access their inner structure either for simple anatomical analysis or for further processing (e.g., histochemistry, immunohistochemistry, in situ hybridization, enzyme histochemistry).
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Citace poskytuje Crossref.org
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- $a Soukup, Aleš $u Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic. ales.soukup@natur.cuni.cz.
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- $a There are various preparatory techniques for light microscopy permitting access to the inner structure of plant body and its development. Minute objects might be processed as whole-mount preparations, while voluminous ones should be separated into smaller pieces. Here we summarize some of the "classical" techniques to cut more voluminous objects into slices and access their inner structure either for simple anatomical analysis or for further processing (e.g., histochemistry, immunohistochemistry, in situ hybridization, enzyme histochemistry).
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