-
Je něco špatně v tomto záznamu ?
High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy
A. Eichmeier, M. Kominkova, J. Pecenka, P. Kominek,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- nemoci rostlin prevence a kontrola virologie MeSH
- ribavirin farmakologie MeSH
- RNA virová genetika MeSH
- rostlinné viry účinky léků genetika MeSH
- sekvenční analýza DNA MeSH
- Vitis virologie MeSH
- vysoce účinné nukleotidové sekvenování * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. A tissue cultures of the plant were established and plantlets obtained were sanitized using Ribavirin. Three years after sanitation, a small RNA sequencing method for virus detection, targeting 21, 22 and 24 nt-long viral small RNAs (vsRNAs), was used to analyze both the mother plant and the sanitized plants. The results showed that the mother plant was infected by the three mentioned viruses and additionally by two viroids - Hop stunt viroid and Grapevine yellow speckle viroid 1. After Ribavirin treatment, the plants contained only the two viroids, with the complete elimination of all the viruses previously present.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20006539
- 003
- CZ-PrNML
- 005
- 20201103105108.0
- 007
- ta
- 008
- 200511s2019 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jviromet.2019.03.003 $2 doi
- 035 __
- $a (PubMed)30851291
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Eichmeier, Ales $u Mendeleum - Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Valticka 334, Lednice, 691 44, Czech Republic. Electronic address: ales.eichmeier@mendelu.cz.
- 245 10
- $a High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy / $c A. Eichmeier, M. Kominkova, J. Pecenka, P. Kominek,
- 520 9_
- $a This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. A tissue cultures of the plant were established and plantlets obtained were sanitized using Ribavirin. Three years after sanitation, a small RNA sequencing method for virus detection, targeting 21, 22 and 24 nt-long viral small RNAs (vsRNAs), was used to analyze both the mother plant and the sanitized plants. The results showed that the mother plant was infected by the three mentioned viruses and additionally by two viroids - Hop stunt viroid and Grapevine yellow speckle viroid 1. After Ribavirin treatment, the plants contained only the two viroids, with the complete elimination of all the viruses previously present.
- 650 12
- $a vysoce účinné nukleotidové sekvenování $7 D059014
- 650 _2
- $a nemoci rostlin $x prevence a kontrola $x virologie $7 D010935
- 650 _2
- $a rostlinné viry $x účinky léků $x genetika $7 D010942
- 650 _2
- $a RNA virová $x genetika $7 D012367
- 650 _2
- $a ribavirin $x farmakologie $7 D012254
- 650 _2
- $a sekvenční analýza DNA $7 D017422
- 650 _2
- $a Vitis $x virologie $7 D027843
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Komínková, Marcela, $d 1974- $u Crop Research Institute, Drnovska 507, Praha 6, Ruzyne, 161 06, Czech Republic. $7 mzk2009496230
- 700 1_
- $a Pecenka, Jakub $u Mendeleum - Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Valticka 334, Lednice, 691 44, Czech Republic.
- 700 1_
- $a Komínek, Petr, $d 1971- $u Crop Research Institute, Drnovska 507, Praha 6, Ruzyne, 161 06, Czech Republic. $7 uzp2009465510
- 773 0_
- $w MED00003047 $t Journal of virological methods $x 1879-0984 $g Roč. 267, č. - (2019), s. 66-70
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/30851291 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200511 $b ABA008
- 991 __
- $a 20201103105104 $b ABA008
- 999 __
- $a ok $b bmc $g 1525397 $s 1096595
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 267 $c - $d 66-70 $e 20190306 $i 1879-0984 $m Journal of virological methods $n J Virol Methods $x MED00003047
- LZP __
- $a Pubmed-20200511
