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Polymer Cancerostatics Targeted by Recombinant Antibody Fragments to GD2-Positive Tumor Cells
R. Pola, V. Král, SK. Filippov, L. Kaberov, T. Etrych, I. Sieglová, J. Sedláček, M. Fábry, M. Pechar,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NV16-28594A
MZ0
CEP - Centrální evidence projektů
- MeSH
- antitumorózní látky aplikace a dávkování chemie farmakologie MeSH
- bungarotoxiny chemie MeSH
- buňky 3T3 MeSH
- doxorubicin aplikace a dávkování chemie farmakologie MeSH
- gangliosidy imunologie MeSH
- jednořetězcové protilátky chemie imunologie MeSH
- kyseliny polymethakrylové chemie MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- nanokonjugáty chemie MeSH
- proliferace buněk účinky léků MeSH
- vazba proteinů MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A water-soluble polymer cancerostatic actively targeted against cancer cells expressing a disialoganglioside antigen GD2 was designed, synthesized and characterized. A polymer conjugate of an antitumor drug doxorubicin with a N-(2-hydroxypropyl)methacrylamide-based copolymer was specifically targeted against GD2 antigen-positive tumor cells using a recombinant single chain fragment (scFv) of an anti-GD2 monoclonal antibody. The targeting protein ligand was attached to the polymer-drug conjugate either via a covalent bond between the amino groups of the protein using a traditional nonspecific aminolytic reaction with a reactive polymer precursor or via a noncovalent but highly specific interaction between bungarotoxin covalently linked to the polymer and the recombinant scFv modified with a C-terminal bungarotoxin-binding peptide. The GD2 antigen binding activity and GD2-specific cytotoxicity of the targeted noncovalent polymer-scFv complex proved to be superior to the covalent polymer-scFv conjugate.
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- $a 10.1021/acs.biomac.8b01616 $2 doi
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- $a Pola, Robert $u Institute of Macromolecular Chemistry , Czech Academy of Sciences , Heyrovského nám. 2 , 162 06 Prague 6 , Czech Republic.
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- $a Polymer Cancerostatics Targeted by Recombinant Antibody Fragments to GD2-Positive Tumor Cells / $c R. Pola, V. Král, SK. Filippov, L. Kaberov, T. Etrych, I. Sieglová, J. Sedláček, M. Fábry, M. Pechar,
- 520 9_
- $a A water-soluble polymer cancerostatic actively targeted against cancer cells expressing a disialoganglioside antigen GD2 was designed, synthesized and characterized. A polymer conjugate of an antitumor drug doxorubicin with a N-(2-hydroxypropyl)methacrylamide-based copolymer was specifically targeted against GD2 antigen-positive tumor cells using a recombinant single chain fragment (scFv) of an anti-GD2 monoclonal antibody. The targeting protein ligand was attached to the polymer-drug conjugate either via a covalent bond between the amino groups of the protein using a traditional nonspecific aminolytic reaction with a reactive polymer precursor or via a noncovalent but highly specific interaction between bungarotoxin covalently linked to the polymer and the recombinant scFv modified with a C-terminal bungarotoxin-binding peptide. The GD2 antigen binding activity and GD2-specific cytotoxicity of the targeted noncovalent polymer-scFv complex proved to be superior to the covalent polymer-scFv conjugate.
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- $a gangliosidy $x imunologie $7 D005732
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- $a Král, Vlastimil $u Institute of Molecular Genetics , Czech Academy of Sciences , Flemingovo nám. 2 , 166 10 Prague 6 , Czech Republic.
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- $a Filippov, Sergey K $u Institute of Macromolecular Chemistry , Czech Academy of Sciences , Heyrovského nám. 2 , 162 06 Prague 6 , Czech Republic.
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- $a Kaberov, Leonid $u Institute of Macromolecular Chemistry , Czech Academy of Sciences , Heyrovského nám. 2 , 162 06 Prague 6 , Czech Republic.
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- $a Etrych, Tomáš $u Institute of Macromolecular Chemistry , Czech Academy of Sciences , Heyrovského nám. 2 , 162 06 Prague 6 , Czech Republic.
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- $a Sieglová, Irena $u Institute of Molecular Genetics , Czech Academy of Sciences , Flemingovo nám. 2 , 166 10 Prague 6 , Czech Republic.
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- $a Sedláček, Juraj $u Institute of Molecular Genetics , Czech Academy of Sciences , Flemingovo nám. 2 , 166 10 Prague 6 , Czech Republic.
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- $a Fábry, Milan $u Institute of Molecular Genetics , Czech Academy of Sciences , Flemingovo nám. 2 , 166 10 Prague 6 , Czech Republic.
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- $a Pechar, Michal $u Institute of Macromolecular Chemistry , Czech Academy of Sciences , Heyrovského nám. 2 , 162 06 Prague 6 , Czech Republic.
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- GRA __
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