• Something wrong with this record ?

Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

M. Horčičková, Š. Čondlová, N. Holubová, B. Sak, D. Květoňová, L. Hlásková, R. Konečný, F. Sedláček, M. Clark, C. Giddings, J. McEvoy, M. Kváč,

. 2019 ; 146 (2) : 220-233. [pub] 20180717

Language English Country Great Britain

Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20006805

Grant support
R15 AI122152 NIAID NIH HHS - United States

Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 µm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 µm). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20006805
003      
CZ-PrNML
005      
20200525092826.0
007      
ta
008      
200511s2019 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1017/S0031182018001142 $2 doi
035    __
$a (PubMed)30012231
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Horčičková, Michaela $u Faculty of Agriculture,University of South Bohemia in České Budějovice,Czech Republic.
245    10
$a Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae) / $c M. Horčičková, Š. Čondlová, N. Holubová, B. Sak, D. Květoňová, L. Hlásková, R. Konečný, F. Sedláček, M. Clark, C. Giddings, J. McEvoy, M. Kváč,
520    9_
$a Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 µm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 µm). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.
650    _2
$a zvířata $7 D000818
650    _2
$a Arvicolinae $x parazitologie $7 D003411
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a kur domácí $7 D002645
650    _2
$a kryptosporidióza $x epidemiologie $x parazitologie $x přenos $7 D003457
650    _2
$a Cryptosporidium $x klasifikace $x genetika $x ultrastruktura $7 D003458
650    _2
$a protozoální DNA $x chemie $x genetika $x izolace a purifikace $7 D016054
650    _2
$a feces $x parazitologie $7 D005243
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a gastrointestinální trakt $x parazitologie $x patologie $x ultrastruktura $7 D041981
650    _2
$a genetická variace $7 D014644
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a mikroskopie elektronová rastrovací $7 D008855
650    _2
$a fluorescenční mikroskopie $7 D008856
650    _2
$a interferenční mikroskopie $7 D008857
650    _2
$a Murinae $7 D051189
650    _2
$a fylogeneze $7 D010802
650    _2
$a polymerázová řetězová reakce $7 D016133
650    _2
$a prevalence $7 D015995
650    _2
$a RNA ribozomální $x genetika $7 D012335
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a nemoci hlodavců $x epidemiologie $x parazitologie $x přenos $7 D012376
650    _2
$a sekvenční seřazení $x veterinární $7 D016415
651    _2
$a Česká republika $7 D018153
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Čondlová, Šárka $u Faculty of Agriculture,University of South Bohemia in České Budějovice,Czech Republic.
700    1_
$a Holubová, Nikola $u Faculty of Agriculture,University of South Bohemia in České Budějovice,Czech Republic.
700    1_
$a Sak, Bohumil $u Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic,České Budějovice,Czech Republic.
700    1_
$a Květoňová, Dana $u Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic,České Budějovice,Czech Republic.
700    1_
$a Hlásková, Lenka $u Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic,České Budějovice,Czech Republic.
700    1_
$a Konečný, Roman $u Faculty of Agriculture,University of South Bohemia in České Budějovice,Czech Republic.
700    1_
$a Sedláček, František $u Faculty of Science,University of South Bohemia in České Budějovice,Czech Republic.
700    1_
$a Clark, Mark $u Biological Sciences Department,North Dakota State University,Fargo,USA.
700    1_
$a Giddings, Catherine $u Microbiological Sciences Department,North Dakota State University,Fargo,USA.
700    1_
$a McEvoy, John $u Microbiological Sciences Department,North Dakota State University,Fargo,USA.
700    1_
$a Kváč, Martin $u Faculty of Agriculture,University of South Bohemia in České Budějovice,Czech Republic.
773    0_
$w MED00010377 $t Parasitology $x 1469-8161 $g Roč. 146, č. 2 (2019), s. 220-233
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30012231 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20200525092826 $b ABA008
999    __
$a ok $b bmc $g 1525663 $s 1096861
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 146 $c 2 $d 220-233 $e 20180717 $i 1469-8161 $m Parasitology $n Parasitology $x MED00010377
GRA    __
$a R15 AI122152 $p NIAID NIH HHS $2 United States
LZP    __
$a Pubmed-20200511

Find record

Citation metrics

Archiving options