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Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528

SR. Ra, MS. Kim, CI. Paek, YC. Pak, SH. Pak, HB. Pak, KC. Ri,

. 2019 ; 64 (6) : 803-808. [pub] 20190401

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20008025

Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5'-GAATTC-3' in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5'overhang.

Citace poskytuje Crossref.org

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