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Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia
V. Nagyová, A. Nagy, J. Timko,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
NLK
Medline Complete (EBSCOhost)
od 2007-01-01 do Před 1 rokem
Springer Nature OA/Free Journals
od 1928-04-01
- MeSH
- Acanthamoeba klasifikace genetika izolace a purifikace fyziologie MeSH
- akantamébová keratitida parazitologie MeSH
- dospělí MeSH
- fylogeneze MeSH
- genotyp MeSH
- geny rRNA MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- molekulární sekvence - údaje MeSH
- protozoální DNA chemie genetika MeSH
- ribozomální DNA chemie genetika MeSH
- RNA protozoální genetika MeSH
- RNA ribozomální 18S genetika MeSH
- rohovka parazitologie MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
Acanthamoeba keratitis (AK) is a relatively rare disease worldwide. Over the past 10 years, five cases of AK were reported in Slovakia. Four preserved Slovak strains and one strain from the Czech Republic isolated from corneal scrapes of patients with AK are characterised in this study. Genotype identification of isolates is based on sequences of the PCR amplimer GTSA.B1 amplified from 18S ribosomal DNA. A strain isolated from the first patient in 1999 was classified as a rare sequence type T15. This is just the second report in which genotype T15 has been associated with AK. The other three Slovak strains were identified as belonging to the most common genotype T4. The only strain originating from the Czech Republic was classified as sporadically appearing sequence type T3. All isolates were also studied for their temperature tolerance and growth characteristics. The cythopatic effect was tested in vitro on Vero cell cultures.
Citace poskytuje Crossref.org
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