BACKGROUND: S100A11 (calgizzarin), a member of the S100 family, is associated with oncogenesis, inflammation and myocardial damage. Our aim was to analyse S100A11 in idiopathic inflammatory myopathies (IIMs) and its association with disease activity features and cancer development. METHODS: S100A11 in muscle was determined by immunohistochemistry in polymyositis (PM), dermatomyositis (DM), myasthenia gravis (MG) and in subjects without autoimmune inflammatory disease (HC). S100A11 in plasma was measured in 110 patients with IIMs (PM, DM, and cancer associated myositis (CAM) patients) and in 42 HC. Disease activity was assessed by myositis disease activity assessment (MYOACT), muscle enzymes and C-reactive protein (CRP) were measured by routine laboratory techniques; autoantibodies by immunoprecipitation or by immunoblot. RESULTS: We observed an accumulation of S100A11 in the cytoplasm of regenerating and necrotizing muscle fibres of PM and DM patients. S100A11 was increased in plasma of all myositis patients compared to HC (3.8 (1.5-16.8) vs 2.8 (1.7-11.2)ng/ml, p=0.011) and in DM and CAM patients compared to HC (4.0 (2.2-14.9) and 4.5 (1.5-9.1) vs 2.8 (1.7-11.2)ng/ml, p<0.001 and p=0.022, respectively). In all myositis patients, S100A11 correlated with the levels of lactate dehydrogenase (r=0.256, p=0.011), aspartate aminotransferase (AST) (r=0.312, p=0.002), CRP (r=0.254, p=0.022) and MYOACT (r=0.245, p=0.022). S100A11 was associated with MYOACT (r=0.377, p=0.030) and pulmonary and cutaneous disease activity in DM patients (r=0.408, p=0.017 and r=0.417, p=0.01, respectively). S100A11 was related to the levels of AST (r=0.412, p=0.027) in PM and to the levels of creatine phosphokinase (r=0.432, p=0.028) in CAM patients. CONCLUSIONS: We show for a first time a potential implication of S100A11 in the local inflammatory and tissue remodelling processes in myositis and an association of circulating S100A11 with disease activity and extra muscular manifestations in DM. Copyright © 2019 Elsevier Ltd. All rights reserved.

Department of Pathology and Molecular Medicine 2nd Medical School and University Hospital Motol Charles University Prague Czech Republic

Institute of Rheumatology and Department of Rheumatology 1st Faculty of Medicine Charles University Prague Czech Republic

Technology Centre of the Czech Academy of Sciences Prague Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc20018621

003      

CZ-PrNML

005      

20220411125155.0

007      

ta

008      

201116s2019 xxk f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.cyto.2018.12.023 $2 doi

035    __

$a (PubMed)30684913

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxk

100    1_

$a Andres Cerezo L $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.

245    10

$a Pro-inflammatory S100A11 is elevated in inflammatory myopathies and reflects disease activity and extramuscular manifestations in myositis / $c Cerezo L Andres, H Hulejova, B Sumova, T Kropackova, O Krystufkova, M Klein, HF Mann, J Zamecnik, O Pecha, K Pavelka, J Vencovsky, L Senolt

520    9_

$a BACKGROUND: S100A11 (calgizzarin), a member of the S100 family, is associated with oncogenesis, inflammation and myocardial damage. Our aim was to analyse S100A11 in idiopathic inflammatory myopathies (IIMs) and its association with disease activity features and cancer development. METHODS: S100A11 in muscle was determined by immunohistochemistry in polymyositis (PM), dermatomyositis (DM), myasthenia gravis (MG) and in subjects without autoimmune inflammatory disease (HC). S100A11 in plasma was measured in 110 patients with IIMs (PM, DM, and cancer associated myositis (CAM) patients) and in 42 HC. Disease activity was assessed by myositis disease activity assessment (MYOACT), muscle enzymes and C-reactive protein (CRP) were measured by routine laboratory techniques; autoantibodies by immunoprecipitation or by immunoblot. RESULTS: We observed an accumulation of S100A11 in the cytoplasm of regenerating and necrotizing muscle fibres of PM and DM patients. S100A11 was increased in plasma of all myositis patients compared to HC (3.8 (1.5-16.8) vs 2.8 (1.7-11.2)ng/ml, p=0.011) and in DM and CAM patients compared to HC (4.0 (2.2-14.9) and 4.5 (1.5-9.1) vs 2.8 (1.7-11.2)ng/ml, p<0.001 and p=0.022, respectively). In all myositis patients, S100A11 correlated with the levels of lactate dehydrogenase (r=0.256, p=0.011), aspartate aminotransferase (AST) (r=0.312, p=0.002), CRP (r=0.254, p=0.022) and MYOACT (r=0.245, p=0.022). S100A11 was associated with MYOACT (r=0.377, p=0.030) and pulmonary and cutaneous disease activity in DM patients (r=0.408, p=0.017 and r=0.417, p=0.01, respectively). S100A11 was related to the levels of AST (r=0.412, p=0.027) in PM and to the levels of creatine phosphokinase (r=0.432, p=0.028) in CAM patients. CONCLUSIONS: We show for a first time a potential implication of S100A11 in the local inflammatory and tissue remodelling processes in myositis and an association of circulating S100A11 with disease activity and extra muscular manifestations in DM.<ovid:br/><ovid:br/> Copyright &#xa9; 2019 Elsevier Ltd. All rights reserved.

650    02

$a dospělí $7 D000328

650    02

$a senioři $7 D000368

650    02

$a senioři nad 80 let $7 D000369

650    02

$a autoprotilátky $x imunologie $7 D001323

650    02

$a autoimunitní nemoci $x imunologie $x patologie $7 D001327

650    02

$a C-reaktivní protein $x analýza $7 D002097

650    02

$a ženské pohlaví $7 D005260

650    02

$a lidé $7 D006801

650    02

$a mužské pohlaví $7 D008297

650    02

$a lidé středního věku $7 D008875

650    12

$a kosterní svalová vlákna $x patologie $7 D018485

650    12

$a polymyozitida $x imunologie $x patologie $7 D017285

650    12

$a proteiny S100 $x metabolismus $7 D009418

700    1_

$a Hulejová, Hana $7 xx0077461 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Šumová, Barbora $7 xx0214573 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Kropáčková, Tereza $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Kryštůfková, Olga, $d 1955- $7 xx0058260 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Klein, Martin $7 xx0092749 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Mann, Heřman, $d 1973- $7 nlk20010095506 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Zámečník, Josef, $d 1974- $7 xx0037787 $u Department of Pathology and Molecular Medicine, 2nd Medical School and University Hospital Motol, Charles University, Prague, Czech Republic

700    1_

$a Pecha, Ondřej $7 _AN084887 $u Technology Centre of the Czech Academy of Sciences, Prague, Czech Republic

700    1_

$a Pavelka, Karel, $d 1954- $7 jn99240000847 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Vencovský, Jiří, $d 1953- $7 jo20000080529 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

700    1_

$a Šenolt, Ladislav, $d 1976- $7 xx0056558 $u Institute of Rheumatology and Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

773    0_

$t Cytokine $g č. 116 (2019), s. 13-20 $p Cytokine $x 1043-4666 $w MED00001313

773    0_

$p Cytokine $g 116:13-20, 2019 04

910    __

$a ABA008 $y p $z 0

990    __

$a 20201116100931 $b ABA008

991    __

$a 20220411125153 $b ABA008

999    __

$a ok $b bmc $g 1583276 $s 1108819

BAS    __

$a 3

BMC    __

$a 2019 $c 116 $d 13-20 $x MED00001313 $i 1043-4666 $m Cytokine (Philadelphia, Pa. Print)

GRA    __

$a NV15-34065A $p MZ0

LZP    __

$a 2020-lp