Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation

V. Fedorova, T. Vanova, L. Elrefae, J. Pospisil, M. Petrasova, V. Kolajova, Z. Hudacova, J. Baniariova, M. Barak, L. Peskova, T. Barta, M. Kaucka, M. Killinger, J. Vecera, O. Bernatik, L. Cajanek, H. Hribkova, D. Bohaciakova,

. 2019 ; 40 (-) : 101563. [pub] 20190829

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20023660

Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular structures called neural rosettes. While a great amount of research has been done using neural rosettes, studies have only inadequately addressed how rosettes are formed and what the molecular mechanisms and pathways involved in their formation are. Here we address this question by detailed analysis of the expression of pluripotency and differentiation-associated proteins during the early onset of differentiation of hPSCs towards neural rosettes. Additionally, we show that the BMP signaling is likely contributing to the formation of the complex cluster of neural rosettes and its inhibition leads to the altered expression of PAX6, SOX2 and SOX1 proteins and the rosette morphology. Finally, we provide evidence that the mechanism of neural rosettes formation in vitro is reminiscent of the process of secondary neurulation rather than that of primary neurulation in vivo. Since secondary neurulation is a largely unexplored process, its understanding will ultimately assist the development of methods to prevent caudal neural tube defects in humans.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20023660
003      
CZ-PrNML
005      
20240625104104.0
007      
ta
008      
201125e20190829xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.scr.2019.101563 $2 doi
035    __
$a (PubMed)31494448
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Fedorova, Veronika $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
245    10
$a Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation / $c V. Fedorova, T. Vanova, L. Elrefae, J. Pospisil, M. Petrasova, V. Kolajova, Z. Hudacova, J. Baniariova, M. Barak, L. Peskova, T. Barta, M. Kaucka, M. Killinger, J. Vecera, O. Bernatik, L. Cajanek, H. Hribkova, D. Bohaciakova,
520    9_
$a Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular structures called neural rosettes. While a great amount of research has been done using neural rosettes, studies have only inadequately addressed how rosettes are formed and what the molecular mechanisms and pathways involved in their formation are. Here we address this question by detailed analysis of the expression of pluripotency and differentiation-associated proteins during the early onset of differentiation of hPSCs towards neural rosettes. Additionally, we show that the BMP signaling is likely contributing to the formation of the complex cluster of neural rosettes and its inhibition leads to the altered expression of PAX6, SOX2 and SOX1 proteins and the rosette morphology. Finally, we provide evidence that the mechanism of neural rosettes formation in vitro is reminiscent of the process of secondary neurulation rather than that of primary neurulation in vivo. Since secondary neurulation is a largely unexplored process, its understanding will ultimately assist the development of methods to prevent caudal neural tube defects in humans.
650    _2
$a COUP transkripční faktor II $x genetika $x metabolismus $7 D051837
650    12
$a buněčná diferenciace $7 D002454
650    _2
$a kultivované buňky $7 D002478
650    _2
$a homeodoménové proteiny $x genetika $x metabolismus $7 D018398
650    _2
$a lidé $7 D006801
650    _2
$a nervové kmenové buňky $x cytologie $x metabolismus $7 D058953
650    _2
$a neurální trubice $x cytologie $x embryologie $x metabolismus $7 D054259
650    12
$a neurulace $7 D054261
650    _2
$a transkripční faktor PAX6 $x genetika $x metabolismus $7 D000071841
650    _2
$a faktory domény POU $x genetika $x metabolismus $7 D050809
650    _2
$a pluripotentní kmenové buňky $x cytologie $x metabolismus $7 D039904
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Vanova, Tereza $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Elrefae, Lina $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Pospisil, Jakub $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Petrasova, Martina $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Kolajova, Veronika $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Hudacova, Zuzana $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Baniariova, Jana $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Barák, Martin $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic. $7 xx0319252
700    1_
$a Peskova, Lucie $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Barta, Tomas $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Kaucka, Marketa $u Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden; Department of Molecular Neurosciences, Medical University of Vienna, Austria.
700    1_
$a Killinger, Michael $u Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Science, Brno, Czech Republic.
700    1_
$a Vecera, Josef $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
700    1_
$a Bernatik, Ondrej $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Cajanek, Lukas $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Hribkova, Hana $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic.
700    1_
$a Bohaciakova, Dasa $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University Brno, Czech Republic. Electronic address: bohaciakova@med.muni.cz.
773    0_
$w MED00167579 $t Stem cell research $x 1876-7753 $g Roč. 40 (20190829), s. 101563
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31494448 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20240625104100 $b ABA008
999    __
$a ok $b bmc $g 1595979 $s 1114336
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 40 $c - $d 101563 $e 20190829 $i 1876-7753 $m Stem cell research $n Stem Cell Res $x MED00167579
LZP    __
$a Pubmed-20201125

Najít záznam

Citační ukazatele

Možnosti archivace