-
Je něco špatně v tomto záznamu ?
Effect of silver nanoparticles conjugated to thiosemicarbazide on biofilm formation and expression of intercellular adhesion molecule genes, icaAD, in Staphylococcus aureus
A. Montazeri, A. Salehzadeh, H. Zamani,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny genetika MeSH
- biofilmy účinky léků MeSH
- kovové nanočástice chemie MeSH
- mikrobiální testy citlivosti MeSH
- molekuly buněčné adheze genetika MeSH
- semikarbazidy farmakologie MeSH
- Staphylococcus aureus účinky léků genetika MeSH
- stříbro farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
Biofilm formation is regarded as an important factor in the establishment of infections caused by Staphylococcus aureus. In the present study, phenotypic and molecular assays were used to evaluate antibiofilm potential of thiosemicarbazide (Tsc) conjugated with silver nanoparticles (Ag NPs) and functionalized by glutamic acid (Ag@Glu/Tsc NPs) against methicillin-resistant S. aureus (MRSA). Ag NPs were synthesized using precipitation method and conjugated to Tsc using glutamic acid. The NPs were characterized using SEM and FTIR spectroscopy analyses. Then, antibiofilm potential of the prepared NPs against MRSA strains was evaluated using phenotypic method and their effects on the expression of biofilm-associated genes icaA and icaD. Finally, the genes involved with the synthesis of intercellular adhesion molecules were determined. According to the results, Ag@Glu/Tsc NPs inhibited biofilm formation of MRSA strains up to 76.7% compared with the control. In addition, expression of the biofilm-associated genes icaA and icaD reduced by 66.7% and 60.3%, respectively in the presence of sub-inhibitory concentration of Ag@Glu/Tsc NPs. In conclusion, Ag@Glu/Tsc NPs could be considered as a potent antibacterial agent to inhibit bacterial biofilms.
Department of Biology Faculty of Science University of Guilan Rasht Iran
Department of Biology Rasht Branch Islamic Azad University Rasht Iran
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20024239
- 003
- CZ-PrNML
- 005
- 20201208102220.0
- 007
- ta
- 008
- 201208s2020 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-019-00715-1 $2 doi
- 035 __
- $a (PubMed)31114932
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Montazeri, Ahmad $u Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.
- 245 10
- $a Effect of silver nanoparticles conjugated to thiosemicarbazide on biofilm formation and expression of intercellular adhesion molecule genes, icaAD, in Staphylococcus aureus / $c A. Montazeri, A. Salehzadeh, H. Zamani,
- 520 9_
- $a Biofilm formation is regarded as an important factor in the establishment of infections caused by Staphylococcus aureus. In the present study, phenotypic and molecular assays were used to evaluate antibiofilm potential of thiosemicarbazide (Tsc) conjugated with silver nanoparticles (Ag NPs) and functionalized by glutamic acid (Ag@Glu/Tsc NPs) against methicillin-resistant S. aureus (MRSA). Ag NPs were synthesized using precipitation method and conjugated to Tsc using glutamic acid. The NPs were characterized using SEM and FTIR spectroscopy analyses. Then, antibiofilm potential of the prepared NPs against MRSA strains was evaluated using phenotypic method and their effects on the expression of biofilm-associated genes icaA and icaD. Finally, the genes involved with the synthesis of intercellular adhesion molecules were determined. According to the results, Ag@Glu/Tsc NPs inhibited biofilm formation of MRSA strains up to 76.7% compared with the control. In addition, expression of the biofilm-associated genes icaA and icaD reduced by 66.7% and 60.3%, respectively in the presence of sub-inhibitory concentration of Ag@Glu/Tsc NPs. In conclusion, Ag@Glu/Tsc NPs could be considered as a potent antibacterial agent to inhibit bacterial biofilms.
- 650 _2
- $a antibakteriální látky $x farmakologie $7 D000900
- 650 _2
- $a biofilmy $x účinky léků $7 D018441
- 650 _2
- $a molekuly buněčné adheze $x genetika $7 D015815
- 650 _2
- $a bakteriální geny $x genetika $7 D005798
- 650 _2
- $a kovové nanočástice $x chemie $7 D053768
- 650 _2
- $a mikrobiální testy citlivosti $7 D008826
- 650 _2
- $a semikarbazidy $x farmakologie $7 D012663
- 650 _2
- $a stříbro $x farmakologie $7 D012834
- 650 _2
- $a Staphylococcus aureus $x účinky léků $x genetika $7 D013211
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Salehzadeh, Ali $u Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran. Salehzadehmb@yahoo.com.
- 700 1_
- $a Zamani, Hojjatolah $u Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 65, č. 1 (2020), s. 153-160
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31114932 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20201208 $b ABA008
- 991 __
- $a 20201208102216 $b ABA008
- 999 __
- $a ok $b bmc $g 1593194 $s 1114920
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 65 $c 1 $d 153-160 $e 20190521 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20201208
