-
Je něco špatně v tomto záznamu ?
Antioxidant properties and neuroprotective effects of Esc-1GN through the regulation of MAPK and AKT signaling
Q. Zeng, B. Zeng, J. Chai, J. Wu, R. Guo, Y. Gao, X. Han, J. Yang, M. Kotsyfakis, X. Xu,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- antioxidancia farmakologie MeSH
- MAP kinasový signální systém MeSH
- myši MeSH
- neuroprotektivní látky farmakologie MeSH
- protoonkogenní proteiny c-akt metabolismus MeSH
- signální transdukce * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
AIMS: This study aimed to explore the antioxidant properties and neuroprotective effects of Esc-1GN. MAIN METHODS: FRAP assay and ABTS, DPPH, and NO radicals radical scavenging assays were performed to investigated the Antioxidant activities of Esc-1GN in vitro. Hydrogen peroxide (H2O2)-induced cell damage model was used to determine the neuroprotective effects of Esc-1GN. Carrageenan-injected inflamed paw model was performed to analysis the antioxidant and anti-inflammatory properties of Esc-1GN in vivo. KEY FINDINGS: Esc-1GN scavenged the ABTS, DPPH, and NO radicals and reduced Fe3+ in a concentration-dependent manner. Moreover, Esc-1GN exhibited neuroprotective activity by decreasing malondialdehyde and reactive oxygen species accumulation, restoring endogenous antioxidant enzyme activity, and inhibiting H2O2-induced cell cycle arrest and apoptosis in PC12 cells. Esc-1GN significantly reversed the dysregulation of MAPK, AKT and NF-κB signaling caused by H2O2. In vivo, Esc-1GN decreased MDA, COX-2, NO, TNF-α, IL-6, and Il-1β levels and increased SOD, CAT activity and GSH level in carrageenan-injected inflamed paw tissues. SIGNIFICANCE: These findings suggest that Esc-1GN might serve as a potential antioxidant agent with therapeutic potential in human neurodegenerative diseases.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20024850
- 003
- CZ-PrNML
- 005
- 20201222160014.0
- 007
- ta
- 008
- 201125s2020 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.lfs.2020.117753 $2 doi
- 035 __
- $a (PubMed)32387419
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Zeng, Qingye $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 245 10
- $a Antioxidant properties and neuroprotective effects of Esc-1GN through the regulation of MAPK and AKT signaling / $c Q. Zeng, B. Zeng, J. Chai, J. Wu, R. Guo, Y. Gao, X. Han, J. Yang, M. Kotsyfakis, X. Xu,
- 520 9_
- $a AIMS: This study aimed to explore the antioxidant properties and neuroprotective effects of Esc-1GN. MAIN METHODS: FRAP assay and ABTS, DPPH, and NO radicals radical scavenging assays were performed to investigated the Antioxidant activities of Esc-1GN in vitro. Hydrogen peroxide (H2O2)-induced cell damage model was used to determine the neuroprotective effects of Esc-1GN. Carrageenan-injected inflamed paw model was performed to analysis the antioxidant and anti-inflammatory properties of Esc-1GN in vivo. KEY FINDINGS: Esc-1GN scavenged the ABTS, DPPH, and NO radicals and reduced Fe3+ in a concentration-dependent manner. Moreover, Esc-1GN exhibited neuroprotective activity by decreasing malondialdehyde and reactive oxygen species accumulation, restoring endogenous antioxidant enzyme activity, and inhibiting H2O2-induced cell cycle arrest and apoptosis in PC12 cells. Esc-1GN significantly reversed the dysregulation of MAPK, AKT and NF-κB signaling caused by H2O2. In vivo, Esc-1GN decreased MDA, COX-2, NO, TNF-α, IL-6, and Il-1β levels and increased SOD, CAT activity and GSH level in carrageenan-injected inflamed paw tissues. SIGNIFICANCE: These findings suggest that Esc-1GN might serve as a potential antioxidant agent with therapeutic potential in human neurodegenerative diseases.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a antioxidancia $x farmakologie $7 D000975
- 650 _2
- $a MAP kinasový signální systém $7 D020935
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a neuroprotektivní látky $x farmakologie $7 D018696
- 650 _2
- $a protoonkogenní proteiny c-akt $x metabolismus $7 D051057
- 650 12
- $a signální transdukce $7 D015398
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Zeng, Baishuang $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Chai, Jinwei $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Wu, Jiena $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Guo, Ruiyin $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Gao, Yahua $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Han, Xiaoyan $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Yang, Jie $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
- 700 1_
- $a Kotsyfakis, Michail $u Institute of Parasitology, Biology Center, Czech Academy of Sciences, Budweis, Czech Republic.
- 700 1_
- $a Xu, Xueqing $u Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address: xu2003@smu.edu.cn.
- 773 0_
- $w MED00003147 $t Life sciences $x 1879-0631 $g Roč. 254, č. - (2020), s. 117753
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32387419 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20201125 $b ABA008
- 991 __
- $a 20201222160011 $b ABA008
- 999 __
- $a ok $b bmc $g 1598995 $s 1115536
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 254 $c - $d 117753 $e 20200506 $i 1879-0631 $m Life sciences $n Life Sci $x MED00003147
- LZP __
- $a Pubmed-20201125
