-
Something wrong with this record ?
Detection of high PBDD/Fs levels and dioxin-like activity in toys using a combination of GC-HRMS, rat-based and human-based DR CALUX® reporter gene assays
C. Budin, J. Petrlik, J. Strakova, S. Hamm, B. Beeler, P. Behnisch, H. Besselink, B. van der Burg, A. Brouwer,
Language English Country Great Britain
Document type Journal Article
- MeSH
- Biological Assay MeSH
- Cell Line MeSH
- Child MeSH
- Play and Playthings * MeSH
- Rats MeSH
- Environmental Pollutants analysis toxicity MeSH
- Humans MeSH
- Luciferases genetics MeSH
- Environmental Monitoring methods MeSH
- Plastics chemistry standards MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Polychlorinated Dibenzodioxins analysis toxicity MeSH
- Dibenzofurans, Polychlorinated analysis toxicity MeSH
- Child, Preschool MeSH
- Receptors, Aryl Hydrocarbon genetics MeSH
- Genes, Reporter MeSH
- Flame Retardants analysis toxicity MeSH
- Transfection MeSH
- Animals MeSH
- Check Tag
- Child MeSH
- Rats MeSH
- Humans MeSH
- Child, Preschool MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from plastics containing certain brominated flame retardants. PBDD/Fs are known ligands for the aryl hydrocarbon receptor (AhR) but are not yet considered in the hazard assessment of dioxin mixtures. The aim of the present study was to determine if PBDD/Fs levels present in plastic constituents of toys could pose a threat to children's health. PBDD/Fs, unlike their chlorinated counterparts (PCDD/Fs), have not been officially assigned toxic equivalence factors (TEFs) by the WHO therefore, we determined their relative potency towards AhR activation in both human and rodent cell-based DR CALUX® bioassays. This allowed us to compare GC-HRMS PBDD/F congener levels, converted to total Toxic Equivalents (TEQ) by using the PCDD/F TEFs, to CALUX Bioanalytical Equivalents (BEQ) levels present in contaminated plastic constituents from children's toys. Finally, an estimate was made of the daily ingestion of TEQs from PBDD/Fs-contaminated plastic toys by child mouthing habits. It is observed that the daily ingestion of PBDD/Fs from contaminated plastic toys may significantly contribute to the total dioxin daily intake of young children.
Arnika Toxics and Waste Programme Delnicka 13 Prague Czech Republic
BioDetection Systems B 5 Science Park 406 1098XH Amsterdam the Netherlands
Mas |münsteranalytical solutions gmbh Wilhelm Schickard Strasse 5 48149 Münster Germany
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20024917
- 003
- CZ-PrNML
- 005
- 20201222153616.0
- 007
- ta
- 008
- 201125s2020 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.chemosphere.2020.126579 $2 doi
- 035 __
- $a (PubMed)32443226
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Budin, Clémence $u VU Amsterdam, Faculty of Sciences, Department of Animal Ecology, De Boelelaan, 1080HV, Amsterdam, the Netherlands; BioDetection Systems B.V., Science Park 406, 1098XH, Amsterdam, the Netherlands. Electronic address: c.n.a.budin@vu.nl.
- 245 10
- $a Detection of high PBDD/Fs levels and dioxin-like activity in toys using a combination of GC-HRMS, rat-based and human-based DR CALUX® reporter gene assays / $c C. Budin, J. Petrlik, J. Strakova, S. Hamm, B. Beeler, P. Behnisch, H. Besselink, B. van der Burg, A. Brouwer,
- 520 9_
- $a Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from plastics containing certain brominated flame retardants. PBDD/Fs are known ligands for the aryl hydrocarbon receptor (AhR) but are not yet considered in the hazard assessment of dioxin mixtures. The aim of the present study was to determine if PBDD/Fs levels present in plastic constituents of toys could pose a threat to children's health. PBDD/Fs, unlike their chlorinated counterparts (PCDD/Fs), have not been officially assigned toxic equivalence factors (TEFs) by the WHO therefore, we determined their relative potency towards AhR activation in both human and rodent cell-based DR CALUX® bioassays. This allowed us to compare GC-HRMS PBDD/F congener levels, converted to total Toxic Equivalents (TEQ) by using the PCDD/F TEFs, to CALUX Bioanalytical Equivalents (BEQ) levels present in contaminated plastic constituents from children's toys. Finally, an estimate was made of the daily ingestion of TEQs from PBDD/Fs-contaminated plastic toys by child mouthing habits. It is observed that the daily ingestion of PBDD/Fs from contaminated plastic toys may significantly contribute to the total dioxin daily intake of young children.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biotest $7 D001681
- 650 _2
- $a buněčné linie $7 D002460
- 650 _2
- $a dítě $7 D002648
- 650 _2
- $a předškolní dítě $7 D002675
- 650 _2
- $a polychlorované dibenzofurany $x analýza $x toxicita $7 D000072338
- 650 _2
- $a monitorování životního prostředí $x metody $7 D004784
- 650 _2
- $a látky znečišťující životní prostředí $x analýza $x toxicita $7 D004785
- 650 _2
- $a retardanty hoření $x analýza $x toxicita $7 D005411
- 650 _2
- $a plynová chromatografie s hmotnostně spektrometrickou detekcí $7 D008401
- 650 _2
- $a reportérové geny $7 D017930
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a luciferasy $x genetika $7 D008156
- 650 _2
- $a plastické hmoty $x chemie $x normy $7 D010969
- 650 12
- $a hra a hračky $7 D010988
- 650 _2
- $a polychlorované dibenzodioxiny $x analýza $x toxicita $7 D000072317
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a receptory aromatických uhlovodíků $x genetika $7 D018336
- 650 _2
- $a transfekce $7 D014162
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Petrlik, Jindrich $u Arnika - Toxics and Waste Programme, Delnicka 13, Prague, Czech Republic. Electronic address: jindrich.petrlik@arnika.org.
- 700 1_
- $a Strakova, Jitka $u Arnika - Toxics and Waste Programme, Delnicka 13, Prague, Czech Republic. Electronic address: jitka.strakova@arnika.org.
- 700 1_
- $a Hamm, Stephan $u Mas |münsteranalytical solutions gmbh, Wilhelm-Schickard-Strasse 5, 48149, Münster, Germany. Electronic address: s.hamm@mas-tp.com.
- 700 1_
- $a Beeler, Bjorn $u IPEN, Gothenburg, Sweden. Electronic address: BjornBeeler@ipen.org.
- 700 1_
- $a Behnisch, Peter $u BioDetection Systems B.V., Science Park 406, 1098XH, Amsterdam, the Netherlands. Electronic address: Peter.Behnisch@bds.nl.
- 700 1_
- $a Besselink, Harrie $u BioDetection Systems B.V., Science Park 406, 1098XH, Amsterdam, the Netherlands. Electronic address: Harrie.Besselink@bds.nl.
- 700 1_
- $a van der Burg, Bart $u BioDetection Systems B.V., Science Park 406, 1098XH, Amsterdam, the Netherlands. Electronic address: Bart.van.der.Burg@bds.nl.
- 700 1_
- $a Brouwer, Abraham $u VU Amsterdam, Faculty of Sciences, Department of Animal Ecology, De Boelelaan, 1080HV, Amsterdam, the Netherlands; BioDetection Systems B.V., Science Park 406, 1098XH, Amsterdam, the Netherlands. Electronic address: Bram.Brouwer@bds.nl.
- 773 0_
- $w MED00002124 $t Chemosphere $x 1879-1298 $g Roč. 251, č. - (2020), s. 126579
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32443226 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20201125 $b ABA008
- 991 __
- $a 20201222153612 $b ABA008
- 999 __
- $a ok $b bmc $g 1599062 $s 1115603
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 251 $c - $d 126579 $e 20200331 $i 1879-1298 $m Chemosphere $n Chemosphere $x MED00002124
- LZP __
- $a Pubmed-20201125
