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Detection of high PBDD/Fs levels and dioxin-like activity in toys using a combination of GC-HRMS, rat-based and human-based DR CALUX® reporter gene assays
C. Budin, J. Petrlik, J. Strakova, S. Hamm, B. Beeler, P. Behnisch, H. Besselink, B. van der Burg, A. Brouwer,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články
- MeSH
- biotest MeSH
- buněčné linie MeSH
- dítě MeSH
- hra a hračky * MeSH
- krysa rodu rattus MeSH
- látky znečišťující životní prostředí analýza toxicita MeSH
- lidé MeSH
- luciferasy genetika MeSH
- monitorování životního prostředí metody MeSH
- plastické hmoty chemie normy MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- polychlorované dibenzodioxiny analýza toxicita MeSH
- polychlorované dibenzofurany analýza toxicita MeSH
- předškolní dítě MeSH
- receptory aromatických uhlovodíků genetika MeSH
- reportérové geny MeSH
- retardanty hoření analýza toxicita MeSH
- transfekce MeSH
- zvířata MeSH
- Check Tag
- dítě MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- předškolní dítě MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from plastics containing certain brominated flame retardants. PBDD/Fs are known ligands for the aryl hydrocarbon receptor (AhR) but are not yet considered in the hazard assessment of dioxin mixtures. The aim of the present study was to determine if PBDD/Fs levels present in plastic constituents of toys could pose a threat to children's health. PBDD/Fs, unlike their chlorinated counterparts (PCDD/Fs), have not been officially assigned toxic equivalence factors (TEFs) by the WHO therefore, we determined their relative potency towards AhR activation in both human and rodent cell-based DR CALUX® bioassays. This allowed us to compare GC-HRMS PBDD/F congener levels, converted to total Toxic Equivalents (TEQ) by using the PCDD/F TEFs, to CALUX Bioanalytical Equivalents (BEQ) levels present in contaminated plastic constituents from children's toys. Finally, an estimate was made of the daily ingestion of TEQs from PBDD/Fs-contaminated plastic toys by child mouthing habits. It is observed that the daily ingestion of PBDD/Fs from contaminated plastic toys may significantly contribute to the total dioxin daily intake of young children.
Arnika Toxics and Waste Programme Delnicka 13 Prague Czech Republic
BioDetection Systems B 5 Science Park 406 1098XH Amsterdam the Netherlands
Mas |münsteranalytical solutions gmbh Wilhelm Schickard Strasse 5 48149 Münster Germany
Citace poskytuje Crossref.org
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- $a Budin, Clémence $u VU Amsterdam, Faculty of Sciences, Department of Animal Ecology, De Boelelaan, 1080HV, Amsterdam, the Netherlands; BioDetection Systems B.V., Science Park 406, 1098XH, Amsterdam, the Netherlands. Electronic address: c.n.a.budin@vu.nl.
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- $a Detection of high PBDD/Fs levels and dioxin-like activity in toys using a combination of GC-HRMS, rat-based and human-based DR CALUX® reporter gene assays / $c C. Budin, J. Petrlik, J. Strakova, S. Hamm, B. Beeler, P. Behnisch, H. Besselink, B. van der Burg, A. Brouwer,
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- $a Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from plastics containing certain brominated flame retardants. PBDD/Fs are known ligands for the aryl hydrocarbon receptor (AhR) but are not yet considered in the hazard assessment of dioxin mixtures. The aim of the present study was to determine if PBDD/Fs levels present in plastic constituents of toys could pose a threat to children's health. PBDD/Fs, unlike their chlorinated counterparts (PCDD/Fs), have not been officially assigned toxic equivalence factors (TEFs) by the WHO therefore, we determined their relative potency towards AhR activation in both human and rodent cell-based DR CALUX® bioassays. This allowed us to compare GC-HRMS PBDD/F congener levels, converted to total Toxic Equivalents (TEQ) by using the PCDD/F TEFs, to CALUX Bioanalytical Equivalents (BEQ) levels present in contaminated plastic constituents from children's toys. Finally, an estimate was made of the daily ingestion of TEQs from PBDD/Fs-contaminated plastic toys by child mouthing habits. It is observed that the daily ingestion of PBDD/Fs from contaminated plastic toys may significantly contribute to the total dioxin daily intake of young children.
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