• Je něco špatně v tomto záznamu ?

Thiophene-linked tetramethylbodipy-labeled nucleotide for viscosity-sensitive oligonucleotide probes of hybridization and protein-DNA interactions

P. Güixens-Gallardo, J. Humpolickova, SP. Miclea, R. Pohl, T. Kraus, P. Jurkiewicz, M. Hof, M. Hocek,

. 2020 ; 18 (5) : 912-919. [pub] 20200110

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025206

Cytosine 2'-deoxyribonucleoside dCTBdp and its triphosphate (dCTBdpTP) bearing tetramethylated thiophene-bodipy fluorophore attached at position 5 were designed and synthesized. The green fluorescent nucleoside dCTBdp showed a perfect dependence of fluorescence lifetime on the viscosity. The modified triphosphate dCTBdpTP was substrate to several DNA polymerases and was used for in vitro enzymatic synthesis of labeled oligonucleotides (ONs) or DNA by primer extension. The labeled single-stranded ONs showed a significant decrease in mean fluorescence lifetime when hybridized to the complementary strand of DNA or RNA and were also sensitive to mismatches. The labeled dsDNA sensed protein binding (p53), which resulted in the increase of its fluorescence lifetime. The triphosphate dCTBdpTP was transported to live cells where its interactions could be detected by FLIM but it did not show incorporation to genomic DNA in cellulo.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025206
003      
CZ-PrNML
005      
20201222153737.0
007      
ta
008      
201125s2020 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1039/c9ob02634g $2 doi
035    __
$a (PubMed)31919486
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Güixens-Gallardo, Pedro $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz and Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic.
245    10
$a Thiophene-linked tetramethylbodipy-labeled nucleotide for viscosity-sensitive oligonucleotide probes of hybridization and protein-DNA interactions / $c P. Güixens-Gallardo, J. Humpolickova, SP. Miclea, R. Pohl, T. Kraus, P. Jurkiewicz, M. Hof, M. Hocek,
520    9_
$a Cytosine 2'-deoxyribonucleoside dCTBdp and its triphosphate (dCTBdpTP) bearing tetramethylated thiophene-bodipy fluorophore attached at position 5 were designed and synthesized. The green fluorescent nucleoside dCTBdp showed a perfect dependence of fluorescence lifetime on the viscosity. The modified triphosphate dCTBdpTP was substrate to several DNA polymerases and was used for in vitro enzymatic synthesis of labeled oligonucleotides (ONs) or DNA by primer extension. The labeled single-stranded ONs showed a significant decrease in mean fluorescence lifetime when hybridized to the complementary strand of DNA or RNA and were also sensitive to mismatches. The labeled dsDNA sensed protein binding (p53), which resulted in the increase of its fluorescence lifetime. The triphosphate dCTBdpTP was transported to live cells where its interactions could be detected by FLIM but it did not show incorporation to genomic DNA in cellulo.
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a sloučeniny boru $x chemie $7 D001896
650    _2
$a kationty $7 D002412
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a DNA $x metabolismus $7 D004247
650    _2
$a DNA vazebné proteiny $x metabolismus $7 D004268
650    _2
$a DNA-dependentní DNA-polymerasy $x metabolismus $7 D004259
650    _2
$a lidé $7 D006801
650    _2
$a lipidy $x chemie $7 D008055
650    12
$a hybridizace nukleových kyselin $7 D009693
650    _2
$a nukleotidy $x chemická syntéza $x chemie $7 D009711
650    _2
$a oligonukleotidové sondy $x metabolismus $7 D015345
650    _2
$a vazba proteinů $7 D011485
650    _2
$a rozpouštědla $x chemie $7 D012997
650    _2
$a fluorescenční spektrometrie $7 D013050
650    _2
$a teplota $7 D013696
650    _2
$a thiofeny $x chemie $7 D013876
650    _2
$a viskozita $7 D014783
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Humpolickova, Jana $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz.
700    1_
$a Miclea, Sebastian Paul $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz.
700    1_
$a Pohl, Radek $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz.
700    1_
$a Kraus, Tomáš $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz.
700    1_
$a Jurkiewicz, Piotr $u J. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, Dolejskova 3, 18223 Prague 8, Czech Republic.
700    1_
$a Hof, Martin $u J. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, Dolejskova 3, 18223 Prague 8, Czech Republic.
700    1_
$a Hocek, Michal $u Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic. hocek@uochb.cas.cz and Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic.
773    0_
$w MED00007088 $t Organic & biomolecular chemistry $x 1477-0539 $g Roč. 18, č. 5 (2020), s. 912-919
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31919486 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20201222153733 $b ABA008
999    __
$a ok $b bmc $g 1599351 $s 1115892
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 18 $c 5 $d 912-919 $e 20200110 $i 1477-0539 $m Organic & biomolecular chemistry $n Org Biomol Chem $x MED00007088
LZP    __
$a Pubmed-20201125
Zpět

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace