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Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression
P. Bohacova, J. Kossl, M. Hajkova, B. Hermankova, E. Javorkova, A. Zajicova, M. Krulova, V. Holan,
Cellular immunology.
2020 ;
347 (-) :
103987.
[pub] 20190913
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/bmc20025290
- MeSH
- Lymphocyte Activation immunology MeSH
- Hypoxia-Inducible Factor 1, alpha Subunit immunology MeSH
- Forkhead Transcription Factors metabolism MeSH
- Interferon-gamma immunology MeSH
- Interleukin-10 biosynthesis MeSH
- Interleukin-4 immunology MeSH
- Cells, Cultured MeSH
- Lipopolysaccharides immunology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- B-Lymphocytes, Regulatory immunology MeSH
- T-Lymphocytes, Regulatory immunology MeSH
- Th2 Cells immunology MeSH
- Transforming Growth Factor beta immunology MeSH
- GATA3 Transcription Factor metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-γ and decreased in the presence of IL-4 or TGF-β. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.
References provided by Crossref.org
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- $a Bohacova, Pavla $u Department of Transplantation Immunology, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic. Electronic address: pavla.bohacova@iem.cas.cz.
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- $a Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression / $c P. Bohacova, J. Kossl, M. Hajkova, B. Hermankova, E. Javorkova, A. Zajicova, M. Krulova, V. Holan,
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- $a The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-γ and decreased in the presence of IL-4 or TGF-β. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.
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- $a Hajkova, Michaela $u Department of Transplantation Immunology, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic. Electronic address: michaela.hajkova@natur.cuni.cz.
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- $a Hermankova, Barbora $u Department of Transplantation Immunology, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic. Electronic address: barbora.hermankova@iem.cas.cz.
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- $a Holan, Vladimir $u Department of Transplantation Immunology, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic. Electronic address: vladimir.holan@iem.cas.cz.
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