The aim of the present study was to evaluate the biodosimetric potential of peripheral blood lymphocytes, particularly of T-cell subsets (null and T helper) and natural killer cells (NK), upon exposure to gamma irradiation (60Co) in vivo. For this purpose, the change in relative numbers of NK cells and T-lymphocyte subsets, as well as in the H2AX phosphorylation rate, were evaluated as potential early markers of the lymphocytic response to irradiation in vivo. These experiments were performed on a Large White Pig model. As a result, significant but not dose-dependent changes in the proportion of lymphocyte subpopulations (NK cells, null and T helper cells) were found after exposure to ionising radiation in vivo. On the other hand, circulating NK cells showed relatively higher radioresistance capacity when compared to the T-lymphocyte subsets; however, gamma-H2AX expression showed no significant difference between the evaluated lymphocyte subsets.

BD Czechia s r o 162 00 Prague Czech Republic

Department of Immunology and Gnotobiology Institute of Microbiology of the CAS v v i 142 20 Prague Czech Republic

Department of Radiobiology Faculty of Military Health Sciences University of Defence 500 01 Hradec Králové Czech Republic

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$a Andrejsová, Lenka $u Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, 500 01 Hradec Králové, Czech Republic.

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$a IN VIVO BIODOSIMETRY OF PORCINE T-LYMPHOCYTE SUBSETS AND NK CELLS / $c L. Andrejsová, Z. Šinkorová, J. Šinkora, A. Tichý, A. Filipová, M. Němcová, M. Šinkora,

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$a The aim of the present study was to evaluate the biodosimetric potential of peripheral blood lymphocytes, particularly of T-cell subsets (null and T helper) and natural killer cells (NK), upon exposure to gamma irradiation (60Co) in vivo. For this purpose, the change in relative numbers of NK cells and T-lymphocyte subsets, as well as in the H2AX phosphorylation rate, were evaluated as potential early markers of the lymphocytic response to irradiation in vivo. These experiments were performed on a Large White Pig model. As a result, significant but not dose-dependent changes in the proportion of lymphocyte subpopulations (NK cells, null and T helper cells) were found after exposure to ionising radiation in vivo. On the other hand, circulating NK cells showed relatively higher radioresistance capacity when compared to the T-lymphocyte subsets; however, gamma-H2AX expression showed no significant difference between the evaluated lymphocyte subsets.

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$a Šinkorová, Zuzana $u Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, 500 01 Hradec Králové, Czech Republic.

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$a Šinkora, Jiří $u BD Czechia s.r.o., 162 00 Prague, Czech Republic.

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$a Tichý, Aleš $u Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, 500 01 Hradec Králové, Czech Republic.

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$a Filipová, Alžběta $u Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, 500 01 Hradec Králové, Czech Republic.

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$a Šinkora, Marek $u Department of Immunology and Gnotobiology, Institute of Microbiology of the CAS, v.v.i., 142 20 Prague, Czech Republic.

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