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Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering

M. Dziadek, R. Kudlackova, A. Zima, A. Slosarczyk, M. Ziabka, P. Jelen, S. Shkarina, A. Cecilia, M. Zuber, T. Baumbach, MA. Surmeneva, RA. Surmenev, L. Bacakova, K. Cholewa-Kowalska, TEL. Douglas,

. 2019 ; 107 (11) : 2479-2491. [pub] 20190722

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025539

Grantová podpora
Foundation for Polish Science - International
P108/12/G108 Grant Agency of the Czech Republic ("Center of Excellence") - International
#20.1907.2018 Grant of the President of Russian Federation for young researchers - International
2017/27/B/ST8/01173 National Science Centre, Poland - International
11.11.160.365 Polish Ministry for Science and Higher Education - International
Research Foundation Flanders - International
Tomsk Polytechnic University Competitiveness Enhancement Program grant - International

The present work focuses on the development of novel multicomponent organic-inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α-TCP in various concentrations (0-70 wt%) was proposed. The results showed that α-TCP underwent incomplete transformation to calcium-deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30-70 wt% α-TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α-TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14-day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast-like MG-63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.

Citace poskytuje Crossref.org

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