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Conservation of chicken male germline by orthotopic transplantation of primordial germ cells from genetically distant donors†
J. Mucksová, M. Reinišová, J. Kalina, B. Lejčková, J. Hejnar, P. Trefil,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
ProQuest Central od 2017-01-01 do Před 1 rokem
Open Access Digital Library od 1969-04-01
Health & Medicine (ProQuest) od 2017-01-01 do Před 1 rokem
Odkazy
PubMed
30980659
DOI
10.1093/biolre/ioz064
Knihovny.cz E-zdroje
- MeSH
- chiméra genetika MeSH
- chov metody MeSH
- křížení genetické MeSH
- kultivované buňky MeSH
- kur domácí * klasifikace genetika MeSH
- kuřecí embryo MeSH
- ohrožené druhy MeSH
- spermatogeneze fyziologie MeSH
- spermie cytologie transplantace MeSH
- testis cytologie MeSH
- transplantace heterologní veterinární MeSH
- zachování plodnosti metody veterinární MeSH
- zachování přírodních zdrojů * metody MeSH
- zárodečné buňky transplantace MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Successful derivation and cultivation of primordial germ cells (PGCs) opened the way to efficient transgenesis and genome editing in the chicken. Furthermore, implantation of male PGCs from non-chicken galliform species into the chicken embryos resulted in cross-species germline chimeras and viable offspring. We have recently improved the PGC technology by demonstrating that chicken male PGCs transplanted into the testes of adult cockerel recipients mature into functional sperms. However, the availability of this orthotopic transplantation for cross-species transfer remains to be explored. Here we tested the capacity of genetically distant male PGCs to mature in the microenvironment of adult testes. We derived PGCs from the Chinese black-bone Silkie and transplanted them into infertile White Leghorn cockerels. Within 15-18 weeks after transplantation, we observed restoration of spermatogenesis in recipient cockerels and production of healthy progeny derived from the transplanted PGCs. Our findings also indicate the possibility of cross-species orthotopic transplantation of PGCs. Thus, our results might contribute to the preservation of endangered avian species and maintaining the genetic variability of the domestic chicken.
BIOPHARM Research Institute of Biopharmacy and Veterinary Drugs Jílové u Prahy Czech Republic
Institute of Molecular Genetics Czech Academy of Sciences Prague Czech Republic
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