• Je něco špatně v tomto záznamu ?

Changes in antioxidant, inflammatory and metabolic markers during 1 week cultivation of human skin explants

J. Vostálová, A. Galandáková, B. Zálešák, R. Lichnovská, K. Čížková, J. Ulrichová, A. Rajnochová Svobodová,

. 2019 ; 39 (5) : 773-782. [pub] 20190103

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025876

Grantová podpora
IGA_LF_2017_011 Faculty of Medicine and Dentistry, Palacký University in Olomouc - International
IGA_LF_2018_012 Faculty of Medicine and Dentistry, Palacký University in Olomouc - International
GACR 15-10897S Grantová Agentura České Republiky - International
RVO 61989592 Universita Palackého v Olomouci - International
RVO 61989592 Institutional Support of Palacký University in Olomouc - International
LO1304 Ministry of Education, Youth and Sports of the Czech Republic - International

Human skin explant (HSE) seems to be a useful model for dermatological/cosmetic testing. HSE prepared from donor superfluous skin from plastic surgery operations is cheap and easily obtainable compared to reconstructed models. The HSE use, however, may be limited by the degeneration processes during cultivation. The aim was to monitor changes in metabolic activity and selected apoptotic, inflammatory and antioxidant parameters during 7 day cultivation. The significant changes were found in the superoxide dismutase-2 level from day 5, glutathione S-reductase level from day 6, metabolic activity and fibulin-5 level from day 4, cyclooxygenase-2, interleukin-6 and interleukin-10 from day 1 to 2. Other selected markers (lipid peroxidation products and glutathione level, glutathione S-transferase, catalase, superoxide dismutase and glutathione S-reductase activity, glutathione peroxidase and glutathione S-reductase levels) were not modified significantly due to high inter-individual variability of skin donors. The HSE microstructure as well as cytokeratin-10 and proliferation marker Ki67 expression was also only minimally affected during cultivation. Collectively, the results demonstrate that HSE represents a good model for short-term studies focused on the physical and chemical agent toxicity, protective potential of compounds or metabolic biotransformation. However, reduced metabolic activity, increased inflammation and the high inter-individual variability and sensitivity of donors have to be taken into consideration.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025876
003      
CZ-PrNML
005      
20201222154133.0
007      
ta
008      
201125s2019 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1002/jat.3766 $2 doi
035    __
$a (PubMed)30604520
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Vostálová, Jitka $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
245    10
$a Changes in antioxidant, inflammatory and metabolic markers during 1 week cultivation of human skin explants / $c J. Vostálová, A. Galandáková, B. Zálešák, R. Lichnovská, K. Čížková, J. Ulrichová, A. Rajnochová Svobodová,
520    9_
$a Human skin explant (HSE) seems to be a useful model for dermatological/cosmetic testing. HSE prepared from donor superfluous skin from plastic surgery operations is cheap and easily obtainable compared to reconstructed models. The HSE use, however, may be limited by the degeneration processes during cultivation. The aim was to monitor changes in metabolic activity and selected apoptotic, inflammatory and antioxidant parameters during 7 day cultivation. The significant changes were found in the superoxide dismutase-2 level from day 5, glutathione S-reductase level from day 6, metabolic activity and fibulin-5 level from day 4, cyclooxygenase-2, interleukin-6 and interleukin-10 from day 1 to 2. Other selected markers (lipid peroxidation products and glutathione level, glutathione S-transferase, catalase, superoxide dismutase and glutathione S-reductase activity, glutathione peroxidase and glutathione S-reductase levels) were not modified significantly due to high inter-individual variability of skin donors. The HSE microstructure as well as cytokeratin-10 and proliferation marker Ki67 expression was also only minimally affected during cultivation. Collectively, the results demonstrate that HSE represents a good model for short-term studies focused on the physical and chemical agent toxicity, protective potential of compounds or metabolic biotransformation. However, reduced metabolic activity, increased inflammation and the high inter-individual variability and sensitivity of donors have to be taken into consideration.
650    _2
$a antioxidancia $x metabolismus $7 D000975
650    _2
$a biologické markery $x metabolismus $7 D015415
650    _2
$a cyklooxygenasa 2 $x metabolismus $7 D051546
650    _2
$a glutathionperoxidasa $x metabolismus $7 D005979
650    _2
$a glutathionreduktasa $x metabolismus $7 D005980
650    _2
$a lidé $7 D006801
650    _2
$a interleukin-6 $x metabolismus $7 D015850
650    _2
$a biologické modely $7 D008954
650    12
$a kůže $x imunologie $x metabolismus $x patologie $7 D012867
650    _2
$a superoxiddismutasa $x metabolismus $7 D013482
650    _2
$a časové faktory $7 D013997
650    _2
$a techniky tkáňových kultur $x metody $7 D046509
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Galandáková, Adéla $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
700    1_
$a Zálešák, Bohumil $u Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, I. P. Pavlova 6, 779 00, Olomouc, Czech Republic.
700    1_
$a Lichnovská, Radka $u Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
700    1_
$a Čížková, Kateřina $u Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
700    1_
$a Ulrichová, Jitka $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
700    1_
$a Rajnochová Svobodová, Alena $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic. Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry,, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.
773    0_
$w MED00008416 $t Journal of applied toxicology : JAT $x 1099-1263 $g Roč. 39, č. 5 (2019), s. 773-782
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30604520 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20201222154129 $b ABA008
999    __
$a ok $b bmc $g 1600021 $s 1116562
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 39 $c 5 $d 773-782 $e 20190103 $i 1099-1263 $m JAT. Journal of applied toxicology $n J Appl Toxicol $x MED00008416
GRA    __
$a IGA_LF_2017_011 $p Faculty of Medicine and Dentistry, Palacký University in Olomouc $2 International
GRA    __
$a IGA_LF_2018_012 $p Faculty of Medicine and Dentistry, Palacký University in Olomouc $2 International
GRA    __
$a GACR 15-10897S $p Grantová Agentura České Republiky $2 International
GRA    __
$a RVO 61989592 $p Universita Palackého v Olomouci $2 International
GRA    __
$a RVO 61989592 $p Institutional Support of Palacký University in Olomouc $2 International
GRA    __
$a LO1304 $p Ministry of Education, Youth and Sports of the Czech Republic $2 International
LZP    __
$a Pubmed-20201125

Najít záznam

Citační ukazatele

Možnosti archivace