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Comparison of the immunomodulatory effect of single MSC batches versus pooled MSC products
L. Hejretová, M. Čedíková, M. Dolejšová, T. Vlas, P. Jindra, D. Lysák, M. Holubová,
Jazyk angličtina Země Nizozemsko
Typ dokumentu srovnávací studie, časopisecké články
Grantová podpora
00669806
Ministerstvo Zdravotnictví Ceské Republiky
NLK
ProQuest Central
od 2000-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2011-02-01 do Před 1 rokem
Nursing & Allied Health Database (ProQuest)
od 2000-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2000-01-01 do Před 1 rokem
- MeSH
- aktivace lymfocytů MeSH
- dárci tkání MeSH
- imunomodulace * MeSH
- kokultivační techniky MeSH
- kultivované buňky MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie imunologie MeSH
- nemoc štěpu proti hostiteli etiologie imunologie terapie MeSH
- proliferace buněk MeSH
- separace buněk MeSH
- T-lymfocyty cytologie imunologie MeSH
- transplantace hematopoetických kmenových buněk škodlivé účinky MeSH
- transplantace mezenchymálních kmenových buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Severe corticosteroid-refractory graft-versus-host-disease (GVHD) is a major non-relapse cause of mortality and morbidity after an allogeneic hematopoietic stem cell transplantation (allo-HSCT). One of the most promising treatment options is using advanced therapy medicinal products based on mesenchymal stem cells (MSCs) immunomodulation ability. The protocols of MSC application differ in many parameters including a source of MSC, a dose, a number of doses or way of preparation of the medicinal product. The process is limited by the need for laborious and expensive manufacturing processes fraught with batch-to-batch variability. In our study, we compared the immunomodulatory effects of different MSC batches versus pooled MSC, specifically the influence on lymphocyte proliferation, the metabolic activity, and the expression of activation markers on T cells. Our goal was to determine whether the effect depends on donor-to-donor heterogeneity and if pooling of MSCs could increase their immunomodulatory ability. All tested batches showed an immunomodulatory effect, with no significant differences between the groups. Our study suggests that immunosuppressive potential is comparable in single batches and pooled products, and the use of products got from individual donors is suitable to treat corticosteroid-refractory GVHD.
Citace poskytuje Crossref.org
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- $a Hejretová, L $u Department of Haematology and Oncology, University Hospital, Pilsen, Alej Svobody 80, 304 60, Pilsen, Czech Republic. Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
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- $a Comparison of the immunomodulatory effect of single MSC batches versus pooled MSC products / $c L. Hejretová, M. Čedíková, M. Dolejšová, T. Vlas, P. Jindra, D. Lysák, M. Holubová,
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- $a Severe corticosteroid-refractory graft-versus-host-disease (GVHD) is a major non-relapse cause of mortality and morbidity after an allogeneic hematopoietic stem cell transplantation (allo-HSCT). One of the most promising treatment options is using advanced therapy medicinal products based on mesenchymal stem cells (MSCs) immunomodulation ability. The protocols of MSC application differ in many parameters including a source of MSC, a dose, a number of doses or way of preparation of the medicinal product. The process is limited by the need for laborious and expensive manufacturing processes fraught with batch-to-batch variability. In our study, we compared the immunomodulatory effects of different MSC batches versus pooled MSC, specifically the influence on lymphocyte proliferation, the metabolic activity, and the expression of activation markers on T cells. Our goal was to determine whether the effect depends on donor-to-donor heterogeneity and if pooling of MSCs could increase their immunomodulatory ability. All tested batches showed an immunomodulatory effect, with no significant differences between the groups. Our study suggests that immunosuppressive potential is comparable in single batches and pooled products, and the use of products got from individual donors is suitable to treat corticosteroid-refractory GVHD.
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