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Simple Syntheses of New Pegylated Trehalose Derivatives as a Chemical Tool for Potential Evaluation of Cryoprotectant Effects on Cell Membrane
K. Pomeisl, J. Richter, M. Golan, I. Kratochvílová,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Grantová podpora
MEYS SAFMAT CZ.02.1.01/0.0/0.0/16_013/0001406, LO1409
Ministerstvo Školství, Mládeže a Tělovýchovy
NLK
Directory of Open Access Journals
od 1997
Free Medical Journals
od 1997
PubMed Central
od 2001
Europe PubMed Central
od 2001
ProQuest Central
od 1997-01-01
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 2009-03-01
Health & Medicine (ProQuest)
od 1997-01-01
- MeSH
- buněčná membrána účinky léků metabolismus MeSH
- click chemie MeSH
- dimethylsulfoxid farmakologie MeSH
- kryoprezervace MeSH
- kryoprotektivní látky farmakologie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- polyethylenglykoly chemie farmakologie MeSH
- průtoková cytometrie MeSH
- trehalosa analogy a deriváty chemická syntéza chemie farmakologie MeSH
- viabilita buněk účinky léků MeSH
- zmrazování MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
In our work, we developed the synthesis of new polyfunctional pegylated trehalose derivatives and evaluated their cryoprotective effect using flow cytometry. We showed that new compounds (modified trehaloses) bound to appropriate extracellular polymeric cryoprotectants could be helpful as a chemical tool for the evaluation of their potential toxic cell membrane influences. Our aim was to form a chemical tool for the evaluation of cryoprotectant cell membrane influences, which are still not easily predicted during the freezing/thawing process. We combined two basic cryoprotectants: polyethyleneglycols (PEGs) and trehalose in the new chemical compounds-pegylated trehalose hybrids. If PEG and trehalose are chemically bound and trehalose is adsorbed on the cell surface PEGs molecules which are, due to the chemical bonding with trehalose, close to the cell surface, can remove the cell surface hydration layer which destabilizes the cell membrane. This was confirmed by the comparison of new material, PEG, trehalose, and their mixture cryoprotective capabilities.
Citace poskytuje Crossref.org
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