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Electrochemical Reduction and Oxidation of Eight Unnatural 2'-Deoxynucleosides at a Pyrolytic Graphite Electrode
J. Špaček, N. Karalkar, M. Fojta, J. Wang, SA. Benner,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
R01 GM128186
NIGMS NIH HHS - United States
- Publikační typ
- časopisecké články MeSH
Recently we showed the reduction and oxidation of six natural 2'-deoxynucleosides in the presence of the ambient oxygen using the very broad potential window of a pyrolytic graphite electrode (PGE). Using the same procedure, 2'-deoxynucleoside analogs (dNs) that are parts of an artificially expanded genetic information system (AEGIS) were analyzed. Seven of the eight tested AEGIS dNs provided specific signals (voltammetric redox peaks). These signals, described here for the first time, will be used in future work to analyze DNA built from expanded genetic alphabets, helping to further develop AEGIS technology and its applications. Comparison of the electrochemical behavior of unnatural dNs with the previously documented behaviors of natural dNs also provides insights into the mechanisms of their respective redox processes.
Department of NanoEngineering University of California San Diego La Jolla CA 92093 USA
Foundation for Applied Molecular Evolution 13709 Progress Blvd Alachua FL 32615 USA
Citace poskytuje Crossref.org
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- $a Špaček, Jan $u Department of Biophysical Chemistry and Molecular Oncology Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65 Brno, The Czech Republic. Department of NanoEngineering, University of California San Diego, La Jolla, CA 92093, USA. Foundation for Applied Molecular Evolution, 13709 Progress Blvd., Alachua, FL 32615, USA.
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- $a Recently we showed the reduction and oxidation of six natural 2'-deoxynucleosides in the presence of the ambient oxygen using the very broad potential window of a pyrolytic graphite electrode (PGE). Using the same procedure, 2'-deoxynucleoside analogs (dNs) that are parts of an artificially expanded genetic information system (AEGIS) were analyzed. Seven of the eight tested AEGIS dNs provided specific signals (voltammetric redox peaks). These signals, described here for the first time, will be used in future work to analyze DNA built from expanded genetic alphabets, helping to further develop AEGIS technology and its applications. Comparison of the electrochemical behavior of unnatural dNs with the previously documented behaviors of natural dNs also provides insights into the mechanisms of their respective redox processes.
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