Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats

P. Krupa, K. Stepankova, JC. Kwok, JW. Fawcett, V. Cimermanova, P. Jendelova, L. Machova Urdzikova,

. 2020 ; 8 (11) : . [pub] 20201105

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21001811

Grant support
19-10365S Grantová Agentura České Republiky
CZ.02.1.01/0.0./0.0/15_003/0000419 Operational Programme Research, Development and Education in the framework of the project "Center of Reconstructive Neuroscience"
MR/S011110/1 Medical Research Council - United Kingdom
NRB119 International Spinal Research Trust
WFL-UK-008-15 Wings for Life

Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty's catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty ́s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21001811
003      
CZ-PrNML
005      
20240703134802.0
007      
ta
008      
210105s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/biomedicines8110477 $2 doi
035    __
$a (PubMed)33167447
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Krupa, Petr $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic. Department of Neurosurgery, Charles University, Medical Faculty and University Hospital Hradec Králové, Sokolska 581, 50005 Hradec Kralove, Czech Republic.
245    10
$a New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats. / $c P. Krupa, K. Stepankova, JC. Kwok, JW. Fawcett, V. Cimermanova, P. Jendelova, L. Machova Urdzikova,
520    9_
$a Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty's catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty ́s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Stepankova, Katerina $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic. Department of Neuroscience, Charles University, Second Faculty of Medicine, 15006 Prague, Czech Republic.
700    1_
$a Kwok, Jessica Cf $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic. Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
700    1_
$a Fawcett, James W $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic. John van Geest Centre for Brain Repair, Dept. Clinical Neurosciences, University of Cambridge, Cambridge CB2 0PY, UK.
700    1_
$a Cimermanova, Veronika $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic.
700    1_
$a Jendelova, Pavla $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic. Department of Neuroscience, Charles University, Second Faculty of Medicine, 15006 Prague, Czech Republic.
700    1_
$a Machová Urdzíková, Lucia $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic. Department of Neuroscience, Charles University, Second Faculty of Medicine, 15006 Prague, Czech Republic. $7 xx0136406
773    0_
$w MED00205373 $t Biomedicines $x 2227-9059 $g Roč. 8, č. 11 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33167447 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20240703134758 $b ABA008
999    __
$a ind $b bmc $g 1614024 $s 1122095
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 8 $c 11 $e 20201105 $i 2227-9059 $m Biomedicines $n Biomedicines $x MED00205373
GRA    __
$a 19-10365S $p Grantová Agentura České Republiky
GRA    __
$a CZ.02.1.01/0.0./0.0/15_003/0000419 $p Operational Programme Research, Development and Education in the framework of the project "Center of Reconstructive Neuroscience"
GRA    __
$a MR/S011110/1 $p Medical Research Council $2 United Kingdom
GRA    __
$a NRB119 $p International Spinal Research Trust
GRA    __
$a WFL-UK-008-15 $p Wings for Life
LZP    __
$a Pubmed-20210105

Find record

Citation metrics

Archiving options