Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Chronic stress impairs male spermatogenesis function and Nectin-3 protein expression in the testis

T. Li, J. Yao, Q. Zhang, Q. Li, J. Li, X. Wang, W. Li, A. Chen, J. Yan

. 2020 ; 69 (2) : 297-306. [pub] 20200323

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21011349

Chronic stress is a crucial public issue that occurs when a person is repetitively stimulated by various stressors. Previous researches have reported that chronic stress induces spermatogenesis dysfunction in the reproductive system, but its molecular mechanisms remain unclear. The nectin protein family, including nectin-1 to nectin-4, is Ca(2+)-independent immunoglobulin-like cell adhesion molecules, that are widely expressed in the hippocampus, testicular tissue, epithelial cells and other sites. Nectin-3 contributes to the sperm development at the late stage, and the abnormal expression of nectin-3 impairs spermatogenesis. Some recent studies have demonstrated that stress induces a decrease in nectin-3 expression in the hippocampus via corticotropin-releasing hormone (CRH) to corticotropin-releasing hormone receptor 1 (CRHR1) pathway. Here, we tested whether chronic stress also caused a reduction in nectin-3 expression in the testis. We established a chronic social defeat stress paradigm, which provides naturalistic and complex chronic stress inmale C57BL/6 mice. After 25 days of chronic social defeat stress, the mice showed weight loss, thymic atrophy and some other typical symptoms of chronic stress (e.g.anxiety-like behavior and social avoidance behavior). We found gonad atrophy, testicular histological structure changes and semen quality reductions in the stressed mice. The stressed male mice significantly spent more time to impregnate the female mice than the control male mice. Moreover, nectin-3 protein levels in stressed mice were significantly decreased in the testes compared with those in control mice. In addition, we found that the CRHR1 expression level was increased in the testes of stressed mice. Therefore, we demonstrated a decreased level of nectin-3 expression and an increase in CRHR1 expression in the testis after exposure to chronic stress, which may provide a potential therapeutic target for the spermatogenesis dysfunction induced by chronic stress.

Citace poskytuje Crossref.org

Bibliografie atd.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc21011349
003      
CZ-PrNML
005      
20210603093701.0
007      
ta
008      
210420s2020 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.934287 $2 doi
035    __
$a (PubMed)32324042
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Li, T. $u Key Laboratory of Cognition and Personality of the Ministry of Education, Faculty of Psychology, Southwest University, Chongqing, China; Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, China
245    10
$a Chronic stress impairs male spermatogenesis function and Nectin-3 protein expression in the testis / $c T. Li, J. Yao, Q. Zhang, Q. Li, J. Li, X. Wang, W. Li, A. Chen, J. Yan
504    __
$a Literatura
520    9_
$a Chronic stress is a crucial public issue that occurs when a person is repetitively stimulated by various stressors. Previous researches have reported that chronic stress induces spermatogenesis dysfunction in the reproductive system, but its molecular mechanisms remain unclear. The nectin protein family, including nectin-1 to nectin-4, is Ca(2+)-independent immunoglobulin-like cell adhesion molecules, that are widely expressed in the hippocampus, testicular tissue, epithelial cells and other sites. Nectin-3 contributes to the sperm development at the late stage, and the abnormal expression of nectin-3 impairs spermatogenesis. Some recent studies have demonstrated that stress induces a decrease in nectin-3 expression in the hippocampus via corticotropin-releasing hormone (CRH) to corticotropin-releasing hormone receptor 1 (CRHR1) pathway. Here, we tested whether chronic stress also caused a reduction in nectin-3 expression in the testis. We established a chronic social defeat stress paradigm, which provides naturalistic and complex chronic stress inmale C57BL/6 mice. After 25 days of chronic social defeat stress, the mice showed weight loss, thymic atrophy and some other typical symptoms of chronic stress (e.g.anxiety-like behavior and social avoidance behavior). We found gonad atrophy, testicular histological structure changes and semen quality reductions in the stressed mice. The stressed male mice significantly spent more time to impregnate the female mice than the control male mice. Moreover, nectin-3 protein levels in stressed mice were significantly decreased in the testes compared with those in control mice. In addition, we found that the CRHR1 expression level was increased in the testes of stressed mice. Therefore, we demonstrated a decreased level of nectin-3 expression and an increase in CRHR1 expression in the testis after exposure to chronic stress, which may provide a potential therapeutic target for the spermatogenesis dysfunction induced by chronic stress.
650    _2
$a zvířata $7 D000818
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a nektiny $x biosyntéza $7 D000075983
650    _2
$a receptory hormonu uvolňujícího kortikotropin $x biosyntéza $x metabolismus $7 D018019
650    _2
$a sociální interakce $7 D000083505
650    _2
$a spermatogeneze $x fyziologie $7 D013091
650    _2
$a psychický stres $x metabolismus $x patofyziologie $7 D013315
650    _2
$a testis $x metabolismus $x patofyziologie $7 D013737
655    _2
$a časopisecké články $7 D016428
700    1_
$a Yao, J. $u Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing, China; Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, China
700    1_
$a Zhang, Q. $u Shigatse Branch, Xinqiao Hospital, Third Military Medical University, Shigatse, China
700    1_
$a Li, Q. $u Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing, China
700    1_
$a Li, J. $u Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, China
700    1_
$a Wang, X. $u Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, China
700    1_
$a Li, W. $u Department of Urology and Nephrology, The Third Affiliated Hospital, Chongqing Medical University, Chongqing, China
700    1_
$a Chen, A. $u Key Laboratory of Cognition and Personality of the Ministry of Education, Faculty of Psychology,Southwest University, Chongqing, China
700    1_
$a Yan, J. $u Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing, China
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 69, č. 2 (2020), s. 297-306
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32324042 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210519164834 $b ABA008
999    __
$a ok $b bmc $g 1659419 $s 1131728
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 69 $c 2 $d 297-306 $e 20200323 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20210420

Najít záznam

Citační ukazatele

Možnosti archivace