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In Vitro Characterization of Sumoylation of HR Proteins

V. Altmannova, L. Krejci

. 2021 ; 2153 (-) : 483-502. [pub] -

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21011681

Grant support
206292/E/17/Z Wellcome Trust - United Kingdom

In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification.

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$a Krejci, Lumir $u Department of Biology, Masaryk University, Brno, Czech Republic. lkrejci@chemi.muni.cz ; International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic. lkrejci@chemi.muni.cz ; National Center for Biomolecular Research, Masaryk University, Brno, Czech Republic. lkrejci@chemi.muni.cz
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